Bowsher R R, Verburg K M, Henry D P
J Biol Chem. 1983 Oct 25;258(20):12215-20.
Histamine N-methyltransferase is a major enzyme for inactivating histamine in mammalian tissues. Development of a sensitive and specific assay for histamine N-methyltransferase has permitted quantification of this activity in 25 rat tissues. Additionally, renal histamine N-methyltransferase was purified to electrophoretic homogeneity. The pivotal step in the purification scheme was an affinity column procedure which involved the specific elution of histamine N-methyltransferase from DEAE-Sephacel by 1 mM histamine. The purified enzyme has a molecular weight of 33,400 and a narrow pH dependency with an optimum at 8.0-8.25. Specific antibody produced to renal histamine N-methyltransferase also immunoprecipitated the brain enzyme completely, therefore indicating neuronal and non-neuronal histamine N-methyltransferase share common antigenic determinants.
组胺N-甲基转移酶是哺乳动物组织中使组胺失活的一种主要酶。一种灵敏且特异的组胺N-甲基转移酶检测方法的开发使得能够对25种大鼠组织中的这种活性进行定量。此外，肾组胺N-甲基转移酶被纯化至电泳纯。纯化方案中的关键步骤是亲和柱程序，该程序涉及用1 mM组胺从DEAE-琼脂糖凝胶中特异性洗脱组胺N-甲基转移酶。纯化后的酶分子量为33,400，pH依赖性较窄，最适pH为8.0 - 8.25。针对肾组胺N-甲基转移酶产生的特异性抗体也能完全免疫沉淀脑酶，因此表明神经元和非神经元组胺N-甲基转移酶具有共同的抗原决定簇。
