Nishida T, Nakagawa S, Awata T, Nishibayashi C, Manabe R
Jpn J Ophthalmol. 1982;26(4):416-24.
Fibronectin (FN), a glycoprotein present in plasma and the extracellular matrix, has been reported to be effective on corneal trophic ulcer. This study reported a rapid method for preparing purified FN eyedrops from a patient's own plasma. Plasma FN concentration in healthy Japanese was found to be 235 +/- 62 micrograms per ml(age 21-31 years old). Men had a higher plasma FN level than women (264 +/- 62 vs. 206 +/- 44 micrograms/ml). The FN eyedrops were prepared by gelatin-coupled Sepharose 4B affinity chromatography and gel filtration by Sephadex G-25 (PD-10). Biochemical and immunochemical analyses revealed that the prepared FN eyedrops contained electrophoretically pure FN and no other plasma proteins were detected. Average FN concentration in the eyedrops was 425 micrograms per ml and recovery rate was 54%. The FN eyedrops were found to be stable at room temperature up to 10 days in biological activity as far as we examined. The total time required to prepare the FN eyedrops was within 2 hours. Thus it is possible to apply them to outpatients as well as inpatients.
纤连蛋白（FN）是一种存在于血浆和细胞外基质中的糖蛋白，据报道对角膜营养性溃疡有效。本研究报告了一种从患者自身血浆中制备纯化FN眼药水的快速方法。发现健康日本人群的血浆FN浓度为每毫升235±62微克（年龄21 - 31岁）。男性的血浆FN水平高于女性（264±62对206±44微克/毫升）。通过明胶偶联的琼脂糖凝胶4B亲和层析和葡聚糖凝胶G - 25（PD - 10）凝胶过滤制备FN眼药水。生化和免疫化学分析表明，制备的FN眼药水含有电泳纯的FN，未检测到其他血浆蛋白。眼药水中FN的平均浓度为每毫升425微克，回收率为54%。据我们检测，FN眼药水在室温下长达10天生物活性稳定。制备FN眼药水所需的总时间在2小时以内。因此，将其应用于门诊患者和住院患者都是可行的。
