Allan I, Pearce J H
J Gen Microbiol. 1983 Jul;129(7):1991-2000. doi: 10.1099/00221287-129-7-1991.
The effect of omission of individual amino acids from growth medium on the multiplication of Chlamydia psittaci (strain guinea pig inclusion conjunctivitis) in cycloheximide-treated McCoy cells has been examined. Marked differences were observed in the amounts of particular amino acids required for normal chlamydial multiplication: omission of either leucine, phenylalanine or valine completely inhibited multiplication, whereas absence of any one of another 10 amino acids had no effect on numbers of cells infected. Threshold concentrations of 80, 80 and approx. 8 nmol ml-1 for leucine, valine and phenylalanine, respectively, were needed for normal chlamydial multiplication. These requirements could not be related either to unusually high content in the whole organism, to degradation in the medium, or, from studies with leucine, to deficient association of leucine with host cells. Leucine deprivation at late stages of the developmental cycle also appeared to regulate multiplication. Possible mechanisms responsible for these effects are discussed.
研究了从生长培养基中去除个别氨基酸对鹦鹉热衣原体（豚鼠包涵体结膜炎菌株）在经放线菌酮处理的 McCoy 细胞中增殖的影响。观察到衣原体正常增殖所需的特定氨基酸量存在显著差异：去除亮氨酸、苯丙氨酸或缬氨酸会完全抑制增殖，而去除另外 10 种氨基酸中的任何一种对感染细胞数量没有影响。衣原体正常增殖分别需要亮氨酸、缬氨酸和苯丙氨酸的阈值浓度约为 80、80 和 8 nmol/ml。这些需求与整个生物体中异常高的含量、培养基中的降解无关，从亮氨酸的研究来看，也与亮氨酸与宿主细胞的缺乏结合无关。发育周期后期的亮氨酸剥夺似乎也调节增殖。讨论了造成这些影响的可能机制。
