Coles A M, Pearce J H
Department of Microbiology, University of Birmingham, UK.
J Gen Microbiol. 1987 Mar;133(3):701-8. doi: 10.1099/00221287-133-3-701.
Chlamydiae have amino acid requirements for growth in tissue culture as defined by those amino acids whose individual omission from the growth medium prevents chlamydial multiplication. We have tested the hypothesis that this inhibition of growth arises as a result of antagonism between particular amino acids such that inhibition occurs when the concentration of one amino acid is reduced in the presence of the antagonist amino acid at high concentration. Using the Chlamydia psittaci strain guinea pig inclusion conjunctivitis (GPIC), in the presence of cycloheximide, the requirement for valine was abrogated by the simultaneous omission of isoleucine, that for phenylalanine by simultaneous omission of tryptophan and that for leucine by simultaneous omission of isoleucine plus valine. The antagonism shown between leucine and isoleucine plus valine appears to be unique among bacteria. In the absence of cycloheximide, GPIC had an additional need for tryptophan, tyrosine and isoleucine; these amino acid requirements were shown for both infected McCoy, HeLa and BHK cells. The results are consistent with a mechanism for regulation of parasite growth which depends on the balance of amino acid concentrations in the extracellular environment.
衣原体在组织培养中生长需要氨基酸，这些氨基酸的定义是：从生长培养基中单独去除它们会阻止衣原体繁殖。我们检验了这样一个假说：这种生长抑制是由于特定氨基酸之间的拮抗作用导致的，即当一种氨基酸的浓度在高浓度拮抗氨基酸存在时降低，就会发生抑制。使用鹦鹉热衣原体豚鼠包涵体结膜炎（GPIC）菌株，在放线菌酮存在的情况下，同时去除异亮氨酸可消除对缬氨酸的需求，同时去除色氨酸可消除对苯丙氨酸的需求，同时去除异亮氨酸和缬氨酸可消除对亮氨酸的需求。亮氨酸与异亮氨酸加缬氨酸之间显示出的拮抗作用在细菌中似乎是独特的。在没有放线菌酮的情况下，GPIC对色氨酸、酪氨酸和异亮氨酸有额外需求；在感染的 McCoy、HeLa 和 BHK 细胞中均显示出这些氨基酸需求。结果与一种依赖于细胞外环境中氨基酸浓度平衡的寄生虫生长调节机制一致。
