Kabir S
J Gen Microbiol. 1983 Jul;129(7):2199-206. doi: 10.1099/00221287-129-7-2199.
The serological properties of cell surface proteins of Vibrio cholerae belonging to both the biotypes (classical and El Tor) and the serotypes (Ogawa and Inaba) were investigated. Proteins were isolated by extracting V. cholerae with EDTA in the presence of sodium chloride. The surface localization of these proteins was confirmed with (a) radioiodinated protein A as an immunoprobe and (b) antiserum absorption studies with whole bacteria. There were similarities among the polypeptides of cell surface proteins isolated from various V. cholerae types. Antisera to these proteins agglutinated several V. cholerae strains, irrespective of biotype, serotype and antibiotic sensitivity. The antisera did not agglutinate pathogenic enteric bacteria such as enterotoxigenic Escherichia coli, Salmonella typhi, Shigella spp., Aeromonas hydrophila and Yersinia enterocolitica. The cell surface proteins of V. cholerae were immunogenic in rabbits as high titres of anti-protein specific antibodies were detected by the ELISA technique in the immune sera. These results suggest that the cell surface proteins are common antigens of V. cholerae and can be developed as a potential vaccine candidate against cholera.
对霍乱弧菌两种生物型（古典生物型和埃尔托生物型）及两种血清型（小川型和稻叶型）的细胞表面蛋白的血清学特性进行了研究。在氯化钠存在的情况下，用乙二胺四乙酸（EDTA）提取霍乱弧菌来分离蛋白质。这些蛋白质的表面定位通过以下方法得以证实：（a）使用放射性碘化蛋白A作为免疫探针；（b）对全菌进行抗血清吸收研究。从不同类型的霍乱弧菌中分离出的细胞表面蛋白的多肽之间存在相似性。针对这些蛋白质的抗血清能凝集多种霍乱弧菌菌株，而不论其生物型、血清型及抗生素敏感性如何。该抗血清不能凝集致病性肠道细菌，如产肠毒素大肠杆菌、伤寒沙门氏菌、志贺氏菌属、嗜水气单胞菌和小肠结肠炎耶尔森氏菌。通过酶联免疫吸附测定（ELISA）技术在免疫血清中检测到高滴度的抗蛋白特异性抗体，表明霍乱弧菌的细胞表面蛋白在兔体内具有免疫原性。这些结果表明，细胞表面蛋白是霍乱弧菌的共同抗原，可开发成为一种潜在的霍乱疫苗候选物。
