Kabir S
J Bacteriol. 1980 Oct;144(1):382-9. doi: 10.1128/jb.144.1.382-389.1980.
The protein compositions of the outer membranes of various Vibrio cholerae strains, belonging to both biotypes (El Tor and classical) and both serotypes (Ogawa and Inaba), were analyzed by electrophoresis on polyacrylamide slab gels in the presence of sodium dodecyl sulfate. All these strains contained a major protein band of molecular weight 48,000. But they differed in the composition and proportions of minor proteins. The outer membrane protein profile was influenced by the growth medium. A protein band of molecular weight 15,000 was observed in the outer membrane when V. cholerae Ogawa 395 (classical) was grown in peptone-water, whereas a protein of molecular weight 68,000 appeared when it was grown in the synthetic medium. Under anaerobic growth conditions the proportion of the 13,000-molecular-weight protein was greatly enhanced. The outer membrane contained heat-modifiable proteins, as the protein bands with molecular weights 41,000 and 37,000 disappeared when membrane proteins were disaggregated in sodium dodecyl sulfate at or above 60 degrees C. The antisera to the outer membrane proteins of V. cholerae Ogawa 395 (classical) produced immunoprecipitation to the outer membrane proteins of both biotypes and both serotypes. Also, the antisera agglutinated bacteria of both biotypes and both serotypes, suggesting the presence of a common protein antigen in the outer membrane of V. cholerae.
对属于两种生物型（埃尔托生物型和古典生物型）以及两种血清型（小川型和稻叶型）的各种霍乱弧菌菌株外膜的蛋白质组成，在十二烷基硫酸钠存在的情况下，通过聚丙烯酰胺平板凝胶电泳进行了分析。所有这些菌株都含有一条分子量为48,000的主要蛋白带。但它们在次要蛋白质的组成和比例上有所不同。外膜蛋白谱受生长培养基的影响。当霍乱弧菌小川型395（古典生物型）在蛋白胨水中生长时，在外膜中观察到一条分子量为15,000的蛋白带，而当它在合成培养基中生长时，则出现一条分子量为68,000的蛋白带。在厌氧生长条件下，分子量为13,000的蛋白质比例大大增加。外膜含有热可修饰蛋白，因为当膜蛋白在60℃或更高温度下于十二烷基硫酸钠中解离时，分子量为41,000和37,000的蛋白带消失。霍乱弧菌小川型395（古典生物型）外膜蛋白的抗血清对两种生物型和两种血清型的外膜蛋白都产生免疫沉淀。此外，该抗血清凝集两种生物型和两种血清型的细菌，表明霍乱弧菌外膜中存在共同的蛋白抗原。
