Gómez-Barriocanal J, Rodríguez-Borrajo C, Barat A, Ramírez G
Neurosci Lett. 1983 Aug 8;38(3):321-6. doi: 10.1016/0304-3940(83)90389-0.
In chick retina, the tailed 20S molecular form of acetylcholinesterase (A12) is slowly degraded to globular forms after homogenization of the tissue in a buffer-salt-detergent solution, in the absence of EDTA. This process can be stopped by the addition of EDTA to the homogenate, prior to high speed centrifugation; however, longer delays in adding EDTA lead to lower recoveries of tailed enzyme. The rate of degradation of A12 is considerably enhanced by high speed centrifugation of this EDTA-less homogenate prior to addition of EDTA.
在鸡视网膜中，在不含乙二胺四乙酸（EDTA）的缓冲盐洗涤剂溶液中对组织进行匀浆后，尾部的20S分子形式的乙酰胆碱酯酶（A12）会缓慢降解为球状形式。在高速离心之前，向匀浆中添加EDTA可阻止此过程；然而，添加EDTA的延迟时间越长，尾部酶的回收率越低。在添加EDTA之前，对这种不含EDTA的匀浆进行高速离心，可大大提高A12的降解速率。
