Villafruela M J, Barat A, Manrique E, Villa S, Ramírez G
Dev Neurosci. 1981;4(1):25-36. doi: 10.1159/000112738.
The developmental profiles of the enzyme acetylcholinesterase, and of some of its quaternary structural forms, characterized by discrete sedimentation coefficients, have been comparatively analyzed in chick retina and optic tectum, between embryonic day 8 and day 10 after hatching. Four molecular species of AChE have been characterized in both retina and tectum during this developmental period: two of them with sedimentation coefficients of 11S and 6S, accounting together for 94-99% of the AChE activity in the initial homogenate, can be easily extracted by homogenization in a buffer containing 1% Triton X-100 and 1 M NaC1, at 4 degrees C. The other two, however, are not extractable by such treatment, but can be released by collagenase from the residue left after the detergent-salt extraction; they have apparent sedimentation coefficients of 21.5S and 16.5S and represent, together, less than 2% of activity in the initial homogenate. All four forms of the enzyme show distinctive patterns of change during the developmental period considered, with significant differences between retina and tectum. These differences are discussed in the context of the specific roles of retina and tectum in the visual process.
在孵化后胚胎第8天至第10天之间，对鸡视网膜和视顶盖中乙酰胆碱酯酶及其一些具有离散沉降系数特征的四级结构形式的发育概况进行了比较分析。在此发育阶段，视网膜和视顶盖中已鉴定出四种分子形式的乙酰胆碱酯酶：其中两种沉降系数为11S和6S，在初始匀浆中它们共同占乙酰胆碱酯酶活性的94 - 99%，在4℃下，通过在含有1% Triton X - 100和1 M NaCl的缓冲液中匀浆可轻松提取。然而，另外两种不能通过这种处理提取，但可通过胶原酶从去污剂 - 盐提取后留下的残渣中释放出来；它们的表观沉降系数为21.5S和16.5S，总共占初始匀浆中活性的不到2%。在考虑的发育阶段，所有四种形式的酶都表现出独特的变化模式，视网膜和视顶盖之间存在显著差异。在视网膜和视顶盖在视觉过程中的特定作用背景下讨论了这些差异。
