Maksimova G A, Sedova T A, Nikol'skaia V N
Zh Mikrobiol Epidemiol Immunobiol. 1983 Aug(8):93-8.
The degree of purification of chemical typhus vaccine from the ballast proteins of the substrate has been studied by chemical and immunochemical methods. The analysis of 11 production batches of the vaccine and a number of model preparations has revealed that chemical typhus vaccine is completely free from nonprotein nitrogen-containing components and, to a considerable extent, purified from ballast protein, its concentration being 0.033-0.242 mg per vaccination dose, and also possesses a high specific activity (198-1454 antigenic units per mg of protein. Ballast proteins include at least 5 egg antigens. The concentration of one of them varies between 0 and 5 micrograms per dose. A number of recommendations is made; their realization will make it possible to increase the degree of purification of chemical typhus vaccine.
已通过化学和免疫化学方法研究了化学性斑疹伤寒疫苗从底物的杂质蛋白中的纯化程度。对该疫苗的11个生产批次和一些模型制剂的分析表明，化学性斑疹伤寒疫苗完全不含含非蛋白氮的成分，并且在很大程度上从杂质蛋白中纯化出来，其浓度为每接种剂量0.033 - 0.242毫克，还具有高比活性（每毫克蛋白198 - 1454个抗原单位）。杂质蛋白至少包括5种鸡蛋抗原。其中一种的浓度在每剂量0至5微克之间变化。提出了一些建议；实施这些建议将有可能提高化学性斑疹伤寒疫苗的纯化程度。
