[普氏立克次体抗原制剂的生化与免疫化学研究]

[Biochemical and immunochemical study of antigen preparations of Rickettsia prowazekii].

作者信息

Zezerov E G, Loginov V S, Kostiukov V I, Lukin E P, Potryvaeva N V

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1986 Jan(1):77-81.

Abstract

The biochemical and immunochemical study of the qualitative composition of R. prowazekii antigenic preparations, isolated and purified by different methods, indicates that these preparations contain high-molecular polypeptide 3 (133600 D), morphologically linked with the cell membrane of R. prowazekii. The method of adsorption chromatography on calcium phosphate permits obtaining specific rickettsial antigens with a greater degree of purification from ballast admixtures than the methods of acid precipitation and gel filtration on Sephadex G-200.

摘要

对用不同方法分离和纯化的普氏立克次体抗原制剂的定性组成进行的生化和免疫化学研究表明，这些制剂含有高分子量多肽3（133600道尔顿），在形态上与普氏立克次体的细胞膜相连。与酸沉淀法和Sephadex G - 200凝胶过滤法相比，磷酸钙吸附色谱法能从杂质中获得更高纯度的特异性立克次体抗原。