Keammerer D, Mayhall C G, Hall G O, Pesko L J, Thomas R B
Am J Hosp Pharm. 1983 Oct;40(10):1650-3.
Microbial growth patterns were studied in intravenous fat emulsions under conditions that simulated touch contamination before or during administration. Commercially available emulsions of 10% and 20% soybean oil and 10% safflower oil in 500-ml bottles were inoculated with two concentrations of each of four organisms: a coagulase-negative Staphylococcus isolated from a venipuncture site, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. The bottles were kept at room temperature, and samples were taken by direct puncture of the i.v. port at 0, 3, 6, 12, 24, 48, and 72 hours, diluted, and plated. Emulsions were visually inspected daily. Growth of the coagulase-negative Staphylococcus was minimal for 48 hours. E. coli showed substantial growth within 12 hours in all three emulsions. Growth patterns for Ps. aeruginosa were similar in all emulsions, and growth approximated that of E. coli within 48 hours. The growth rate of C. albicans was intermediate between that of Staphylococcus and those of E. coli and Ps. aeruginosa. Growth of C. albicans was greater in 10% safflower oil emulsion than in the other emulsions. No physical changes were observed. The coagulase-negative Staphylococcus showed less growth than C. albicans, Ps. aeruginosa, and E. coli. Substantial growth within 12 hours was seen only with E. coli. C. albicans exhibited preferential growth in 10% safflower oil emulsion.
在模拟给药前或给药期间接触污染的条件下，研究了静脉脂肪乳剂中的微生物生长模式。将市售的500毫升瓶装10%和20%大豆油以及10%红花油乳剂接种四种微生物的两种浓度：一种从静脉穿刺部位分离出的凝固酶阴性葡萄球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌。将瓶子置于室温下，在0、3、6、12、24、48和72小时通过直接穿刺静脉输液端口采集样本，稀释后进行平板接种。每天对乳剂进行目视检查。凝固酶阴性葡萄球菌在48小时内生长极少。大肠杆菌在所有三种乳剂中12小时内均有大量生长。所有乳剂中铜绿假单胞菌的生长模式相似，48小时内生长情况与大肠杆菌相近。白色念珠菌的生长速率介于葡萄球菌与大肠杆菌和铜绿假单胞菌之间。白色念珠菌在10%红花油乳剂中的生长比在其他乳剂中更旺盛。未观察到物理变化。凝固酶阴性葡萄球菌的生长比白色念珠菌、铜绿假单胞菌和大肠杆菌少。仅大肠杆菌在12小时内有大量生长。白色念珠菌在10%红花油乳剂中表现出优先生长。
