Dawson T M, Wamsley J K
Brain Res Bull. 1983 Sep;11(3):325-34. doi: 10.1016/0361-9230(83)90169-7.
Localization of the binding sites for a radioactive form of the tricyclic antidepressant imipramine has been accomplished using receptor autoradiographic techniques. These studies demonstrated a marked overlap between regions presenting dense concentrations of imipramine binding sites and regions known to receive serotonergic projections. Lesions of the serotonergic neurons were created by intracerebroventricular injections of the potent neurotoxin 5,7-dihydroxytryptamine. The effectiveness of these lesions was ascertained by immunohistochemical localization of serotonin containing neurons in both lesioned and vehicle treated control animals. Animals lesioned in this fashion, showed a dramatic loss of imipramine binding sites in all of the areas which were previously densely labeled. The catecholamine containing neurons were lesioned in another group of animals using 6-hydroxydopamine. The effects of these lesions were ascertained using fluorescence histochemical techniques. In these animals, the imipramine binding sites were unaltered. These results suggest that imipramine binding sites are presynaptic and are located on serotonin containing terminals and not on catecholamine containing neurons.
利用受体放射自显影技术已完成了对三环类抗抑郁药丙咪嗪的放射性形式结合位点的定位。这些研究表明，呈现密集丙咪嗪结合位点的区域与已知接受5-羟色胺能投射的区域之间存在明显重叠。通过脑室内注射强效神经毒素5,7-二羟基色胺来造成5-羟色胺能神经元的损伤。通过对损伤动物和用赋形剂处理的对照动物中含5-羟色胺神经元进行免疫组织化学定位来确定这些损伤的有效性。以这种方式损伤的动物在所有先前被密集标记的区域中显示出丙咪嗪结合位点的显著丧失。在另一组动物中使用6-羟基多巴胺损伤含儿茶酚胺的神经元。利用荧光组织化学技术确定这些损伤的效果。在这些动物中，丙咪嗪结合位点未改变。这些结果表明，丙咪嗪结合位点是突触前的，位于含5-羟色胺的终末上，而不是位于含儿茶酚胺的神经元上。
