重组质粒转化的猴肾细胞大量分泌人生长激素。

Abundant excretion of human growth hormone by recombinant-plasmid-transformed monkey kidney cells.

作者信息

Lupker J H, Roskam W G, Miloux B, Liauzun P, Yaniv M, Jouannau J

出版信息

Gene. 1983 Oct;24(2-3):281-7. doi: 10.1016/0378-1119(83)90088-4.

Abstract

A recombinant plasmid was constructed permitting the efficient synthesis of human growth hormone (hGH) in monkey cells. The plasmid contains the cDNA sequence of the hGH precursor and controlled by the SV40 early promoter, an intron, and the poly(A)-addition site of the mouse alpha-globin gene. To permit selection of transformed cells, a selectable marker (xanthine-guanine phosphoribosyl transferase; XGPRT) was also introduced into this plasmid. Transformation of an established monkey kidney cell line (VERO) permitted the isolation of cell lines excreting hGH. One of these strains (VEH 1) excreted hGH up to 5.6 micrograms per 10(6) cells per 24 h during exponential growth.

摘要

构建了一种重组质粒，可使其在猴细胞中高效合成人生长激素（hGH）。该质粒包含hGH前体的cDNA序列，并由SV40早期启动子、一个内含子以及小鼠α-珠蛋白基因的聚腺苷酸（poly(A)）加尾位点控制。为了能够选择转化细胞，还将一个选择标记（黄嘌呤-鸟嘌呤磷酸核糖转移酶；XGPRT）引入到该质粒中。对已建立的猴肾细胞系（VERO）进行转化，从而分离出分泌hGH的细胞系。其中一个菌株（VEH 1）在指数生长期每24小时每10⁶个细胞可分泌高达5.6微克的hGH。