Pavlakis G N, Hizuka N, Gorden P, Seeburg P, Hamer D H
Proc Natl Acad Sci U S A. 1981 Dec;78(12):7398-402. doi: 10.1073/pnas.78.12.7398.
We have constructed simian virus 40 recombinants carrying two different human growth hormone (hGH) genes. Monkey kidney cells infected with these recombinants synthesize, process, and secrete hGH. The product of gene 1, which has coding sequences identical to those of a cloned hGH complementary DNA, is indistinguishable from pituitary hGH by several criteria. The product of gene 2, which is predicted to encode a variant protein, is less immunoreactive than pituitary hGH but binds efficiently to hGH cell surface receptors. These results show that gene 2 has the potential to be expressed into a previously unidentified form of hGH. They also demonstrate that it is possible to produce a mature hormone by gene transfer in eukaryotic cells and indicate the utility of the simian virus 40-monkey cell system for producing and characterizing secreted animal cell proteins.
我们构建了携带两种不同人类生长激素（hGH）基因的猿猴病毒40重组体。用这些重组体感染猴肾细胞后，细胞会合成、加工并分泌hGH。基因1的产物，其编码序列与克隆的hGH互补DNA的编码序列相同，通过多项标准与垂体hGH无法区分。基因2的产物预计编码一种变体蛋白，其免疫反应性低于垂体hGH，但能有效地与hGH细胞表面受体结合。这些结果表明基因2有潜力表达为一种此前未鉴定的hGH形式。它们还证明了通过真核细胞中的基因转移产生成熟激素是可能的，并表明猿猴病毒40 - 猴细胞系统在生产和鉴定分泌性动物细胞蛋白方面的实用性。
