6-Hydroxydopamine and 5,7-dihydroxytryptamine selectively reduce dopamine and 5-hydroxytryptamine metabolites in cerebroventricular perfusates of rats.

The efflux into the lateral cerebral ventricles of metabolites of dopamine (DA) and 5-hydroxytryptamine (5HT) was determined in unanesthetized rats bearing chronically implanted push-pull cannulae. Pretreatment with 6-hydroxydopamine (6-OHDA) reduced the basal efflux of 3,4-dihydroxyphenylacetic acid (DOPAC) and homovanillic acid (HVA), but not of 5-hydroxyindoleacetic acid (5HIAA). The haloperidol-induced increase in the efflux of DOPAC and HVA was markedly attenuated in the 6-OHDA-pretreated rats. In rats treated with 5,7-hydroxytryptamine (5,7-DHT) the basal efflux of DOPAC and HVA was unaffected, while that of 5HIAA was markedly reduced; in these animals the ability of L-tryptophan to increase the perfusate content of 5HIAA was abolished. These results indicate that metabolites of DA and 5HT appearing in cerebroventricular perfusates of rats originate from DA and 5HT neuronal terminals in the brain.