Nakao M, Nakao T, Komatsu Y, Sano K, Sasakawa S
Biomed Biochim Acta. 1983;42(5):527-35.
In order to manipulate red cells to remove additives and metabolites prior to transfusion after prolonged preservation, osmotic fragility must be depressed. In isoosmotic sucrose solutions, the osmotic hemolysis curve is shifted to the left (less fragile) within several hours at 37 degrees C and within several days at 4 degrees C. However, hyperosmolar solution induced hemolysis when 0.9% NaCl was added after preservation. In isoosmotic sucrose solutions, the order of effectiveness in maintaining intracellular ATP content was: adenine plus inosine greater than inosine alone greater than adenine alone greater than no addition. The same order was also seen in depression of the increase in osmotic fragility during storage for 8 weeks. Formation of too stable erythrocytes to osmotic fragility and accordingly too low filtrability was prevented by the addition of appropriate amounts of physiological saline. Media containing isotonic sucrose, adenine and inosine depressed the increase in osmotic fragility during preservation for 6 weeks at 4 degrees C. ATP and 2,3-DPG levels were also better maintained than in various media containing a small amount of adenine. This finding with the former which indicated that the media containing isoosmotic sucrose, adenine and inosine prolonged posttransfusional viability of rabbit cells very much, suggests that these media are worthy of further investigation, since excess metabolites can be easily removed before transfusion.
为了在长时间保存后输血前对红细胞进行处理以去除添加剂和代谢产物，必须降低其渗透脆性。在等渗蔗糖溶液中，在37℃下数小时内以及在4℃下数天内，渗透溶血曲线会向左移动（脆性降低）。然而，保存后添加0.9%氯化钠时，高渗溶液会诱导溶血。在等渗蔗糖溶液中，维持细胞内ATP含量的有效性顺序为：腺嘌呤加肌苷大于单独的肌苷大于单独的腺嘌呤大于不添加。在储存8周期间渗透脆性增加的降低方面也观察到相同的顺序。通过添加适量的生理盐水可防止形成对渗透脆性过于稳定的红细胞，从而防止过滤性过低。含有等渗蔗糖、腺嘌呤和肌苷的培养基在4℃下保存6周期间可降低渗透脆性的增加。与含有少量腺嘌呤的各种培养基相比，ATP和2,3-DPG水平也能得到更好的维持。这一发现以及之前的研究结果表明，含有等渗蔗糖、腺嘌呤和肌苷的培养基可大大延长兔细胞输血后的活力，这表明这些培养基值得进一步研究，因为在输血前可以很容易地去除多余的代谢产物。
