Koenig H, Goldstone A D, Lu C Y
Biochem Biophys Res Commun. 1983 Nov 15;116(3):1039-48. doi: 10.1016/s0006-291x(83)80247-2.
A focal freeze injury to rat cerebral cortex induces an early (less than 5 min) increase in brain ornithine decarboxylase activity and an accumulation of polyamines involving cerebral microvessels. This polyamine synthesis correlates with the abnormal increase in microvascular permeability, monitored by uptake of Evans Blue and sod. fluorescein. The ornithine decarboxylase inhibitor alpha-difluoromethylornithine suppressed the injury-induced increment in spermidine and spermine and microvascular permeability. Putrescine nullified alpha-difluoromethylornithine inhibition and restored microvessel spermidine and spermine and the pathological increase in microvascular permeability. These results indicate that polyamine synthesis is obligatory for blood-brain barrier breakdown. alpha-Difluoromethylornithine may be useful in the treatment of vasogenic brain edema.
对大鼠大脑皮层进行局灶性冷冻损伤会导致脑鸟氨酸脱羧酶活性早期（不到5分钟）增加，并使涉及脑微血管的多胺积聚。这种多胺合成与微血管通透性的异常增加相关，通过伊文思蓝和磺基荧光素的摄取来监测。鸟氨酸脱羧酶抑制剂α-二氟甲基鸟氨酸抑制了损伤诱导的亚精胺和精胺增加以及微血管通透性增加。腐胺消除了α-二氟甲基鸟氨酸的抑制作用，并恢复了微血管中亚精胺和精胺以及微血管通透性的病理性增加。这些结果表明，多胺合成是血脑屏障破坏所必需的。α-二氟甲基鸟氨酸可能对治疗血管源性脑水肿有用。
