Kirpekar S M, Nobiletti J, Trifaró J M
Br J Pharmacol. 1983 Aug;79(4):947-52. doi: 10.1111/j.1476-5381.1983.tb10540.x.
The effect of 6-hydroxydopamine on the catecholamine content and cell morphology of bovine adrenal chromaffin cells in culture was investigated. 6-Hydroxydopamine markedly reduced the catecholamine content of the cultured chromaffin cells after 6 and 24 h exposure. The effect was dose-related, with half-maximal depletion occurring at 2.4 X 10(-5) M. Cells exposed to 6-hydroxydopamine for 3 h and then to normal medium without the drug for 3 h more showed the same degree of toxicity as cells exposed to the drug for the entire 6 h. Ascorbate at high concentrations also exhibited toxicity toward chromaffin cells between 6 and 24 h of exposure. 6-Hydroxydopamine produced marked changes in cell morphology. At 1 h the cells appeared normal, at 3 h the processes were markedly shortened, and at 6 h they were completely retracted. On exposure for 24 h there were gross morphological changes and most cells were detached and free-floating in the medium. The toxicity of 6-hydroxydopamine in bovine adrenal chromaffin cells is discussed.
研究了6-羟基多巴胺对培养的牛肾上腺嗜铬细胞儿茶酚胺含量和细胞形态的影响。在暴露6小时和24小时后，6-羟基多巴胺显著降低了培养的嗜铬细胞的儿茶酚胺含量。该效应呈剂量依赖性，在2.4×10(-5)M时出现半数最大耗竭。暴露于6-羟基多巴胺3小时，然后再暴露于不含该药物的正常培养基3小时的细胞，与暴露于该药物6小时的细胞表现出相同程度的毒性。高浓度的抗坏血酸在暴露6至24小时之间也对嗜铬细胞表现出毒性。6-羟基多巴胺使细胞形态发生显著变化。1小时时细胞外观正常，3小时时突起明显缩短，6小时时完全缩回。暴露24小时后出现明显的形态变化，大多数细胞脱离并在培养基中自由漂浮。讨论了6-羟基多巴胺在牛肾上腺嗜铬细胞中的毒性。
