Norlund R, Grankvist K, Norlund L, Täljedal I B
Virchows Arch A Pathol Anat Histopathol. 1984;404(1):31-8. doi: 10.1007/BF00704248.
Stereological techniques on electron microscopy micrographs were used to evaluate the morphological changes of cultured islet beta cells that had been exposed to alloxan or 6-hydroxydopamine. Trypan Blue exclusion by cells cultured for 3 days indicated that the cells were 100% viable. Electron microscopy revealed that nearly all of the surviving cultured cells were beta cells. Exposure to 5 mmol/l alloxan or 1-5 mmol/l 6-hydroxydopamine for 10 or 30 min caused a general swelling of the cultured cells with a concomitant swelling of mitochondria and nuclei. The size of the secretory granules was not affected by the drugs. Only 3-10% of the cells excluded Trypan Blue after exposure to 5 mmol/l alloxan or 6-hydroxydopamine. The data conform with the hypothesis that a primary action of alloxan and 6-hydroxydopamine is at the plasma membrane level of beta cells.
运用电子显微镜显微照片的体视学技术，评估暴露于四氧嘧啶或6-羟基多巴胺的培养胰岛β细胞的形态变化。培养3天的细胞经台盼蓝排斥试验表明细胞活力为100%。电子显微镜显示，几乎所有存活的培养细胞都是β细胞。暴露于5 mmol/L四氧嘧啶或1 - 5 mmol/L 6-羟基多巴胺10或30分钟，导致培养细胞普遍肿胀，同时线粒体和细胞核也肿胀。分泌颗粒的大小不受药物影响。暴露于5 mmol/L四氧嘧啶或6-羟基多巴胺后，只有3 - 10%的细胞能排斥台盼蓝。这些数据符合以下假说：四氧嘧啶和6-羟基多巴胺的主要作用位点是β细胞的质膜水平。
