Plant T M, Zorub D S
Endocrinology. 1984 Feb;114(2):560-5. doi: 10.1210/endo-114-2-560.
An earlier finding that the decline in LH and FSH secretion which typically occurs in neonatally orchidectomized rhesus monkeys between 8-32 weeks of age was temporally associated with a broad peak in circulating concentrations of immunoreactive testosterone has prompted us to evaluate the role of adrenal androgens in determining the ontogeny of gonadotropin secretion in this species. To this end, six orchidectomized (1 week) infantile rhesus monkeys were bilaterally adrenalectomized between 2 and 5 weeks of age and subsequently maintained on daily glucocorticoid (cortisone acetate or hydrocortisone acetate) and mineralocorticoid (desoxycorticosterone acetate) replacement. Plasma cortisol concentrations and electrolytes (Na+ and K+) were monitored at approximately weekly intervals, and the steroid replacements were titrated in an attempt to maintain the foregoing parameters within physiological limits. Plasma was also obtained at weekly intervals for 50 weeks in order to determine the time courses of LH and FSH secretion during this phase of postnatal development. Bilateral adrenalectomy resulted in an immediate and permanent decline in plasma testosterone to undetectable concentrations. This procedure together with appropriate steroid replacement, however, did not appear to influence the developmental pattern of gonadotropin secretion in neonatally orchidectomized monkeys; most notably, the reduction in the open loop secretion of LH and FSH between 8-32 weeks of age was neither interrupted nor masked by adrenalectomy. The growth rate of the adrenalectomized monkeys, as reflected by weekly body weight determinations, was less than that of adrenally intact animals. These findings suggest that adrenal androgens are not involved in the initiation of the prepubertal restraint of gonadotropin secretion in the rhesus monkey and are consistent with the notion that the adrenal gland does not play a major role in determining the timing of puberty onset in higher primates.
早期的一项研究发现，新生期切除睾丸的恒河猴在8至32周龄时，促黄体生成素（LH）和促卵泡生成素（FSH）分泌的下降与循环中免疫反应性睾酮浓度的广泛峰值在时间上相关，这促使我们评估肾上腺雄激素在该物种促性腺激素分泌个体发育中的作用。为此，六只在1周龄时切除睾丸的幼年恒河猴在2至5周龄时进行双侧肾上腺切除术，随后每日给予糖皮质激素（醋酸可的松或醋酸氢化可的松）和盐皮质激素（醋酸脱氧皮质酮）替代治疗。大约每周监测一次血浆皮质醇浓度和电解质（Na +和K +），并调整类固醇替代剂量，试图将上述参数维持在生理范围内。为了确定产后发育这一阶段LH和FSH分泌的时间进程，还每隔一周采集50周的血浆。双侧肾上腺切除术导致血浆睾酮立即且永久性下降至无法检测的浓度。然而，该手术连同适当的类固醇替代治疗，似乎并未影响新生期切除睾丸的猴子促性腺激素分泌的发育模式；最值得注意的是，8至32周龄时LH和FSH开放环分泌的减少既未被肾上腺切除术打断，也未被其掩盖。通过每周测定体重反映，肾上腺切除的猴子的生长速度低于肾上腺完整的动物。这些发现表明，肾上腺雄激素不参与恒河猴青春期前促性腺激素分泌抑制的起始过程，这与肾上腺在高等灵长类动物青春期开始时间的决定中不发挥主要作用的观点一致。
