Loveland P M, Coulombe R A, Libbey L M, Pawlowski N E, Sinnhuber R O, Nixon J E, Bailey G S
Food Chem Toxicol. 1983 Oct;21(5):557-62. doi: 10.1016/0278-6915(83)90140-0.
beta-Naphthoflavone (beta NF) fed to rainbow trout (Salmo gairdneri) at 50 or 500 ppm in the diet, modified the in vitro metabolism of aflatoxin B1 (AFB1) by the postmitochondrial fraction (PMF) of the liver. Production of aflatoxicol (AFL) was significantly less in the 500 ppm beta NF-fed group (33.9 ng/mg protein) than in the control group (45.7 ng/mg protein), aflatoxin M1 production was dependent on the dose of beta NF, being greatest in the 500 ppm beta NF-fed group (48.9 ng/mg protein), intermediate in the 50 ppm beta NF-fed group (3.7 ng/mg protein), and was not detected in controls. A new trout metabolite, 4-hydroxyaflatoxicol (aflatoxicol M1, AFLM1) was also detected in small amounts from in vitro metabolism by liver PMF from beta NF-fed trout. Sufficient quantities of AFLM1 for confirmation of identity by ultraviolet spectra, mass spectra and nuclear magnetic resonance spectra were prepared by biotransformation of AFL using liver microsomes and isolation by HPLC. In a modified Ames mutagen assay with Salmonella typhimurium TA98, ALFM1 was 4.1% as mutagenic as AFB1 in a previous determination. The carcinogenicity of AFLM1 to rainbow trout is expected to be considerably less than that of AFB1.
以50 ppm或500 ppm的剂量将β-萘黄酮（β-NF）添加到虹鳟鱼（Salmo gairdneri）的饲料中，会改变肝脏线粒体后成分（PMF）对黄曲霉毒素B1（AFB1）的体外代谢。500 ppm β-NF喂养组中黄曲霉毒素醇（AFL）的产量（33.9 ng/mg蛋白质）显著低于对照组（45.7 ng/mg蛋白质），黄曲霉毒素M1的产量取决于β-NF的剂量，在500 ppm β-NF喂养组中最高（48.9 ng/mg蛋白质），在50 ppm β-NF喂养组中处于中等水平（3.7 ng/mg蛋白质），而在对照组中未检测到。在β-NF喂养的虹鳟鱼肝脏PMF的体外代谢中，还少量检测到一种新的虹鳟鱼代谢产物4-羟基黄曲霉毒素醇（黄曲霉毒素醇M1，AFLM1）。通过使用肝脏微粒体对AFL进行生物转化并用HPLC分离，制备了足够量的AFLM1，用于通过紫外光谱、质谱和核磁共振光谱确认其身份。在一项改良的鼠伤寒沙门氏菌TA98 Ames诱变试验中，ALFM1的诱变性是之前测定中AFB1的4.1%。预计AFLM1对虹鳟鱼的致癌性远低于AFB1。
