Qwarnstrom E E, Omnell K A, Hand A R
J Oral Pathol. 1983 Dec;12(6):417-29. doi: 10.1111/j.1600-0714.1983.tb00354.x.
The recovery of the rat submandibular gland after retrograde infusion of water-soluble radiographic contrast medium was studied using an experimental model. During continuous monitoring of the developing intraglandular pressure, the glands were subjected to ductal and slight parenchymal filling or heavy parenchymal filling with the medium. The animals were killed after varying recovery periods, and the tissue was prepared for light and electron microscopic examination. Dilation of the ductal lumina, induced during ductal and slight parenchymal filling, was successively reduced and, generally, the parenchyma had a normal appearance at 30 h. In glands subjected to heavy parenchymal filling, the changes in the intralobular ducts were more pronounced and were also seen at later times after infusion. Alterations in the acini, comprising fusion of secretory granules, vacuole formation and dilation of the acinar lumina and intercellular canaliculi, were observed. At later times, atrophy of the parenchymal cells occurred together with an apparent proliferation of the connective-tissue stroma, as well as an increase in the number of small blood vessels. An inflammatory cell-infiltrate was seen in both groups of animals, but was most prominent in glands subjected to heavy parenchymal filling. The infiltrate, comprised primarily of macrophages and polymorphonuclear leukocytes, reached a peak at 20 h after infusion. At later times, mast cells and occasional eosinophils were seen. The observed alterations and the pattern of recovery are most likely due to the induced intraglandular pressure and the following inflammatory reaction. It is also possible that the changes, to some extent, are influenced by the presence of the contrast medium in the tissue.
利用实验模型研究了水溶性放射造影剂逆行灌注后大鼠颌下腺的恢复情况。在持续监测腺体内压力变化的过程中，分别用造影剂对腺体进行导管及轻度实质充盈或重度实质充盈。在不同的恢复时间段后处死动物，制备组织用于光镜和电镜检查。导管及轻度实质充盈时诱导产生的导管管腔扩张逐渐减轻，一般在30小时时实质外观正常。在接受重度实质充盈的腺体中，小叶内导管的变化更为明显，且在灌注后更晚的时间仍可见到。观察到腺泡的改变，包括分泌颗粒融合、空泡形成以及腺泡腔和细胞间小管扩张。在后期，实质细胞萎缩，同时结缔组织基质明显增生，小血管数量增加。两组动物均可见炎性细胞浸润，但在重度实质充盈的腺体中最为显著。浸润细胞主要由巨噬细胞和多形核白细胞组成，在灌注后20小时达到高峰。在后期，可见肥大细胞和偶尔的嗜酸性粒细胞。观察到的这些改变及恢复模式很可能是由诱导的腺体内压力及随后的炎症反应所致。造影剂在组织中的存在也有可能在一定程度上影响了这些变化。
