Qwarnstrom E E, Hand A R
J Oral Pathol. 1983 Dec;12(6):430-45. doi: 10.1111/j.1600-0714.1983.tb00355.x.
Lipid-soluble radiographic contrast medium was infused retrogradely through the main excretory duct of the rat submandibular gland until ductal and slight parenchymal filling or heavy parenchymal filling was achieved, as determined by the developing intraglandular pressure. The glandular tissue was fixed by vascular perfusion at different times following infusion, and examined by light and electron microscopy. Dilation of the intralobular ducts and scalloping of the luminal border were pronounced after both degrees of filling. Widening of the acinar lumina and intercellular canaliculi occurred early. Masses of fused secretory granules were commonly seen in the cytoplasm of the acinar cells and formation of vacuoles occurred frequently. In some acinar cells, densely packed granules filled the major part of the cytoplasm. Large lysosomes and autophagic vacuoles, frequently present in the various parenchymal cells, often contained apparent contrast medium. In addition, widening of the intercellular spaces between parenchymal cells was seen, particularly in the acini and intercalated ducts. An inflammatory cell-infiltrate, primarily comprised of polymorphonuclear leukocytes, was particularly prominent at 20 h and 30 h after infusion. The changes were generally more pronounced and persisted for a longer time in glands subject to heavy parenchymal filling. Large cyst-like spaces surrounded by inflammatory cells were seen in the lobules and in the interlobular connective tissue of these glands. At later times, areas containing apparent contrast medium surrounded by macrophages, were frequently observed in the connective-tissue stroma. Atrophy of the parenchymal cells was seen later, after heavy parenchymal filling, and a proliferation of the connective tissue had occurred. The changes were most probably caused by the elevated intraglandular pressure induced during infusion, and the subsequent cell damage and inflammatory reaction. A foreign body reaction, induced by the retained lipid-soluble contrast medium, was probably partly responsible for the morphological alterations observed, following infusion.
通过大鼠下颌下腺的主要排泄导管逆行注入脂溶性放射造影剂，直至根据腺体内压力的变化确定导管和轻度实质充盈或重度实质充盈。在注入后的不同时间通过血管灌注固定腺组织，并进行光镜和电镜检查。两种程度的充盈后，小叶内导管扩张和管腔边界呈扇形都很明显。腺泡腔和细胞间小管早期就出现增宽。在腺泡细胞的细胞质中常见融合的分泌颗粒团块，并且经常出现空泡形成。在一些腺泡细胞中，密集堆积的颗粒充满了大部分细胞质。各种实质细胞中经常出现的大溶酶体和自噬空泡，通常含有明显的造影剂。此外，可见实质细胞之间的细胞间隙增宽，特别是在腺泡和闰管中。主要由多形核白细胞组成的炎性细胞浸润在注入后20小时和30小时尤为明显。在重度实质充盈的腺体中，这些变化通常更明显且持续时间更长。在这些腺体的小叶和小叶间结缔组织中可见被炎性细胞包围的大囊样间隙。在后期，在结缔组织基质中经常观察到被巨噬细胞包围的含有明显造影剂的区域。在重度实质充盈后，后期可见实质细胞萎缩，并且发生了结缔组织增生。这些变化很可能是由注入过程中引起的腺体内压力升高以及随后的细胞损伤和炎症反应所致。注入后观察到的形态学改变可能部分归因于滞留的脂溶性造影剂引起的异物反应。
