de Gomez Dumm I N, de Alaniz M J, Brenner R R
Lipids. 1983 Nov;18(11):781-8. doi: 10.1007/BF02534636.
The effect of different fatty acids supplemented to a fat-free diet on the activity of delta 5 desaturase was studied. Fat-free diet produces a reduction in the conversion of eicosa-8,11,14-trienoic acid to arachidonic acid. The addition of the cis-omega 6 acids, linoleic, gamma-linolenic or arachidonic to the diet produces an increase of eicosatrienoic acid desaturation, shifting delta 5 desaturase activity towards the controls on a balanced diet. This reactivation is apparently produced by induction of enzyme biosynthesis since linoleate effect was suppressed by simultaneous cycloheximide injection. On the contrary, no changes in delta 5 desaturation activity were found when the diet was supplemented with palmitic or 9-trans,12-trans-linoleic acid. The changes on the activity of delta 5 desaturase were compared with the fatty acid composition of plasma and liver microsomes.
研究了在无脂饮食中添加不同脂肪酸对Δ5去饱和酶活性的影响。无脂饮食会降低二十碳-8,11,14-三烯酸向花生四烯酸的转化。向饮食中添加顺式ω-6酸，即亚油酸、γ-亚麻酸或花生四烯酸，会增加二十碳三烯酸的去饱和作用，使Δ5去饱和酶活性向均衡饮食对照组的水平转变。这种再激活显然是由酶生物合成的诱导产生的，因为同时注射环己酰亚胺会抑制亚油酸的作用。相反，当饮食中添加棕榈酸或9-反式,12-反式-亚油酸时，未发现Δ5去饱和活性有变化。将Δ5去饱和酶活性的变化与血浆和肝脏微粒体的脂肪酸组成进行了比较。
