Tegtmeier B R, Andersen B R
Rev Infect Dis. 1983 Nov-Dec;5 Suppl 5:S963-70. doi: 10.1093/clinids/5.supplement_5.s963.
The nature of the enhanced resistance to Pseudomonas aeruginosa sepsis induced by type-specific lipopolysaccharide vaccine was examined in a mouse model of cyclophosphamide-induced granulocytopenia. Mice actively immunized with type-specific vaccine survived significantly longer than did nonimmune mice (P less than .002) when challenged 8, 12, or 16 days after immunization. This protection was nonspecific eight days after immunization and specific 12 days after immunization. Passive immunization of mice with specific antibody resulted in significant, though minimal, protection. In contrast, long-term protection was observed when the passive transfer of specific antibody was combined with nonspecific immunization. This observation suggests that the specific protection observed with type-specific active immunization results from the interaction of specific antibody and an immunization-induced nonspecific cellular effector. While no significant effect of immunization on granulocyte counts in peripheral blood was demonstrated, studies of phagocytosis performed with peritoneal mononuclear cells suggest that the macrophage may be the immunization-induced, nonspecific cellular effector.
在环磷酰胺诱导的粒细胞减少小鼠模型中，研究了型特异性脂多糖疫苗诱导的对铜绿假单胞菌败血症增强抵抗力的性质。在免疫后8、12或16天进行攻击时，用型特异性疫苗主动免疫的小鼠比未免疫小鼠存活时间显著更长（P小于0.002）。这种保护在免疫后8天是非特异性的，在免疫后12天是特异性的。用特异性抗体对小鼠进行被动免疫产生了显著但最小程度的保护。相比之下，当特异性抗体的被动转移与非特异性免疫相结合时，观察到了长期保护。这一观察结果表明，型特异性主动免疫所观察到的特异性保护是由特异性抗体与免疫诱导的非特异性细胞效应物相互作用产生的。虽然未证明免疫对外周血粒细胞计数有显著影响，但对腹膜单核细胞进行的吞噬作用研究表明，巨噬细胞可能是免疫诱导的非特异性细胞效应物。
