Mazurov A V, Leytin V L, Repin V S, Smirnov V N, Förster W
Thromb Res. 1983 Oct 15;32(2):189-205. doi: 10.1016/0049-3848(83)90030-0.
Soluble plasma inducers and inhibitors of platelet activity and fluid dynamics of the blood stream are effective modulators of platelet-vessel wall interactions. Effects of platelet activity inducers, arachidonic acid (AA) and stable prostaglandin endoperoxides analogue (U46619), on platelet disposition on the bottom of multiwell tissue culture plates coated with fibrillar calf skin collagen (CSC) have been studied by scanning electron microscopy (SEM). Both agents stimulate platelet spreading and formation of large surface-bound multilayer (thrombi-like) aggregates on a CSC substrate. AA and U46619 effects on spreading and thrombi-like aggregate formation depend on the speed of platelet suspension shaking during platelet deposition on the surface. In the absence of shaking, both inducers mainly stimulate the spreading of platelets: spread platelets fuse and form widespread sheets covering up to 50% of the CSC-coated surface. An increase in the shaking speed leads to the decrease of the platelet spreading, while the number of surface-bound thrombi-like aggregates grows, reaching the maximum at a shaking speed of 40 back and forth cycles per min. The thrombi-like aggregates mainly consist of fused platelets and always contain the basal sheet of spread platelets, which suggests the participation of the latter in aggregate attachment to the surface. Large aggregates are absent in the population of nonadherent platelets. The obtained data indicate that AA metabolites participate in platelet spreading and thrombi-like aggregate formation, the processes specific for platelet-surface interactions. The use of the suggested model for the in vitro study of platelet spreading and mural thrombi formation, and for screening of antithrombotic and thrombolytic drugs is discussed.
血小板活性的可溶性血浆诱导剂和抑制剂以及血流的流体动力学是血小板与血管壁相互作用的有效调节剂。通过扫描电子显微镜（SEM）研究了血小板活性诱导剂花生四烯酸（AA）和稳定的前列腺素内过氧化物类似物（U46619）对涂有纤维状小牛皮肤胶原蛋白（CSC）的多孔组织培养板底部血小板分布的影响。两种试剂均刺激血小板在CSC底物上的铺展并形成大的表面结合多层（血栓样）聚集体。AA和U46619对铺展和血栓样聚集体形成的影响取决于血小板沉积在表面期间血小板悬液振荡的速度。在没有振荡的情况下，两种诱导剂主要刺激血小板的铺展：铺展的血小板融合并形成覆盖高达50%的CSC包被表面的广泛薄片。振荡速度的增加导致血小板铺展减少，而表面结合的血栓样聚集体的数量增加，在每分钟40个来回循环的振荡速度下达到最大值。血栓样聚集体主要由融合的血小板组成，并且总是包含铺展血小板的基底层，这表明后者参与聚集体与表面的附着。在非粘附血小板群体中不存在大的聚集体。获得的数据表明AA代谢产物参与血小板铺展和血栓样聚集体形成，这是血小板-表面相互作用特有的过程。讨论了所建议的模型在体外研究血小板铺展和壁血栓形成以及筛选抗血栓和溶栓药物中的应用。
