Khabbaz R F, Arnow P M, Highsmith A K, Herwaldt L A, Chou T, Jarvis W R, Lerche N W, Allen J R
Am J Med. 1984 Jan;76(1):62-8. doi: 10.1016/0002-9343(84)90751-4.
In October 1980, two units of blood contaminated with Pseudomonas fluorescens caused septic transfusion reactions in two recipients at a Chicago hospital; one patient died. Both units had been purchased from the same blood center. Investigation at the blood center and at other hospitals it supplied revealed another fatal case of P. fluorescens sepsis that had occurred one year earlier. Despite extensive environmental culturing at the blood center, the source of P. fluorescens was not identified. However, comparison of the interval between collection and administration of contaminated and non-contaminated units indicated that prolonged storage was a risk factor that may have caused clustering of cases in one hospital. Laboratory studies showed that small inocula of P. fluorescens proliferated in refrigerated fresh whole blood and reached 10(6) to 10(7) colony-forming units per milliliter seven days after incubation. These data suggest that prolonged storage of blood may be an important risk factor for the development of transfusion-related sepsis.
1980年10月，两单位被荧光假单胞菌污染的血液在芝加哥一家医院导致两名受血者发生败血症性输血反应；一名患者死亡。这两单位血液均购自同一血液中心。对该血液中心及其供血的其他医院进行调查发现，一年前曾发生过另一例致命的荧光假单胞菌败血症病例。尽管在血液中心进行了广泛的环境培养，但荧光假单胞菌的来源仍未确定。然而，对受污染和未受污染血液单位采集与输注之间的时间间隔进行比较表明，长时间储存是一个危险因素，可能导致了一家医院内病例的聚集。实验室研究表明，荧光假单胞菌的小接种量在冷藏的新鲜全血中增殖，培养七天后每毫升达到10⁶至10⁷菌落形成单位。这些数据表明，血液的长时间储存可能是输血相关败血症发生的一个重要危险因素。
