Puckett A, Davison G, Entwistle C C, Barbara J A
Regional Blood Transfusion Centre, John Radcliffe Hospital, Headington, Oxford.
J Clin Pathol. 1992 Feb;45(2):155-7. doi: 10.1136/jcp.45.2.155.
To determine the prevalence of Pseudomonas fluorescens on the arms of blood donors, and to elucidate one possible cause for its predominance (60% of cases during 1980-89) in exogenous post transfusion septicaemia (PTS).
Skin swabs were taken from the arms of 782 blood donors and cultured on to heated blood agar. After incubation, Oxidase reagent and the Gram stain were used to select non-fermentative Gram negative rods, which were then subcultured and identified using the Analytical Profile System (API) 20 NE system.
Non-fermentative Gram negative rods were found on the arms of 11.7% of donors, Pseudomonas spp on 1.0%, and Ps fluorescens on the arms of 0.3% of donors.
This evidence emphasises the absolute requirement for efficient skin cleansing of blood donors' arms to minimise the risk of exogenous PTS.
确定荧光假单胞菌在献血者手臂上的流行情况，并阐明其在外源性输血后败血症（PTS）中占主导地位（1980 - 1989年期间60%的病例）的一个可能原因。
从782名献血者的手臂上采集皮肤拭子，并接种于加热的血琼脂上。培养后，使用氧化酶试剂和革兰氏染色来筛选非发酵革兰氏阴性杆菌，然后进行传代培养，并使用分析谱系统（API）20 NE系统进行鉴定。
在11.7%的献血者手臂上发现了非发酵革兰氏阴性杆菌，1.0%的手臂上发现了假单胞菌属，0.3%的献血者手臂上发现了荧光假单胞菌。
这一证据强调了对献血者手臂进行有效皮肤清洁的绝对必要性，以将外源性PTS的风险降至最低。
