Chang G N, Gabrielson D A
Exp Parasitol. 1984 Feb;57(1):81-5. doi: 10.1016/0014-4894(84)90066-3.
Serial, in vitro passage of Toxoplasma gondii (Rh strain) was successfully performed in a cell line derived from ovine fetal kidney cells. Invasion of this parasite into the kidney cells was easily discernible 1 hr after inoculation. The subsequent proliferation of the parasite was followed in the cytoplasm of the kidney cells. Very active endodyogeny and rosette formations, as many as 13 in a cell, were observed in the cytoplasm of the kidney cells 48 hr postinoculation. After 96 hr of incubation, the parasite population had increased about 132-fold. The virulence of T. gondii against mice was not attenuated after 2 years of in vitro growth which represented 100 serial passages through the kidney cell cultures. Although no "exotoxin" was produced by T. gondii grown in vitro, a Toxoplasma sp. agar gel immunodiffusion test antigen was isolated from the cell-free supernatant fluid of the kidney cell cultures which was identical to an antigen isolated from "toxogenic" organisms harvested from infected mice.
在源自绵羊胎儿肾细胞的细胞系中成功进行了弓形虫（Rh株）的连续体外传代培养。接种后1小时，这种寄生虫对肾细胞的侵袭很容易辨别。随后在肾细胞的细胞质中观察到了寄生虫的增殖。接种后48小时，在肾细胞的细胞质中观察到非常活跃的内二分裂和玫瑰花结形成，一个细胞中多达13个。孵育96小时后，寄生虫数量增加了约132倍。在体外生长2年（相当于通过肾细胞培养连续传代100次）后，弓形虫对小鼠的毒力并未减弱。虽然体外培养的弓形虫不产生“外毒素”，但从肾细胞培养物的无细胞上清液中分离出一种弓形虫琼脂凝胶免疫扩散试验抗原，它与从感染小鼠体内收获的“产毒”生物体中分离出的一种抗原相同。
