Chang G N, Gabrielson D A
Zhonghua Min Guo Wei Sheng Wu Ji Mian Yi Xue Za Zhi. 1983 Sep;16(3):183-9.
A toxoplasma gondii antigen prepared from the cell-free supernatant of ovine fetal kidney (OFK) cell cultures produced a sharp precipitation line against anti-Toxoplasma serum in an agar-gel immunodiffusion (AGID) test. Results obtained from the AGID and indirect hemagglutination (IHA) tests on 46 human and nine rabbit sera were compared. Thirty-three percent of the serum samples, which were negative for the Toxoplasma IHA test, were positive when assayed by the AGID method. The AGID test appears to be superior to the IHA test for detecting low titers of antibodies against T. gondii. After 70 generations of serial passages through the OFK cell cultures, T. gondii still produced the AGID test antigen.
从绵羊胎肾（OFK）细胞培养物的无细胞上清液中制备的弓形虫抗原，在琼脂凝胶免疫扩散（AGID）试验中与抗弓形虫血清产生了清晰的沉淀线。对46份人血清和9份兔血清进行AGID试验和间接血凝试验（IHA），并比较结果。弓形虫IHA试验阴性的血清样本中，33%用AGID法检测时呈阳性。AGID试验在检测低滴度抗弓形虫抗体方面似乎优于IHA试验。在通过OFK细胞培养物连续传代70代后，弓形虫仍能产生AGID试验抗原。
