Simon M F, Chap H, Douste-Blazy L
FEBS Lett. 1984 Jan 23;166(1):115-9. doi: 10.1016/0014-5793(84)80055-1.
The calcium ionophore properties of A23187 and of two lysophosphatidic acid (LPA) analogs (1-palmitoyl- and 1-hexadecyl-sn-glycero-3-phosphate or P-GPA and H-GPA, respectively) were compared using platelet membrane vesicles loaded with 45Ca. Half maximal effect (HME) was obtained at 5 microM and 10 microM for H-GPA and P-GPA, respectively, against 0.7 microM for A23187, which released 2 times more Ca. The three compounds also induced platelet aggregation with a HME at 0.5 microM, 0.3 microM and 0.01 microM for A23187, P-GPA and H-GPA, respectively. The clear dissociation between the two effects appearing for both LPA raises some doubt about the general idea that (lyso) PA participate in cell activation through their calcium ionophore properties.
使用装载有45Ca的血小板膜囊泡，比较了A23187以及两种溶血磷脂酸（LPA）类似物（分别为1-棕榈酰-sn-甘油-3-磷酸和1-十六烷基-sn-甘油-3-磷酸，即P-GPA和H-GPA）的钙离子载体特性。H-GPA和P-GPA的半数最大效应（HME）分别在5 microM和10 microM时获得，而A23187在0.7 microM时获得HME，其释放的Ca多2倍。这三种化合物还诱导血小板聚集，A23187、P-GPA和H-GPA的HME分别为0.5 microM、0.3 microM和0.01 microM。两种LPA出现的两种效应之间的明显解离，对（溶血）磷脂酸通过其钙离子载体特性参与细胞激活这一普遍观点提出了一些质疑。
