Solomon C, Tuchweber B, Srivastava U, Nadeau M
Mech Ageing Dev. 1984 Jan;24(1):9-27. doi: 10.1016/0047-6374(84)90173-8.
The specific activities of the lysosomal enzymes acid phosphatase, beta-galactosidase, arylsulphatase B and cathepsin D were determined in homogenates of livers of rats fed ad libitum and of rats subjected to long-term dietary restriction (10%, 30% and 50% of diet consumed by the ad libitum group). Dietary restriction began soon after weaning and animals were sacrificed 3, 9, 15 and 24 weeks later. Dietary restriction influenced all four enzymes but the changes depended on the enzyme as well as on the degree and duration of the dietary restriction. Total activity of acid phosphatase increased significantly at 3 weeks of restriction but only in the 50% group. The activity returned to normal values at 9 weeks. Arylsulphatase B increased in all experimental groups with a more pronounced change observed at 3 weeks and in the more severely restricted rats. No notable change in the activities of beta-galactosidase and cathepsin D activities was observed. Changes in the liver ultrastructure paralleled the biochemical changes seen at 3 weeks. Numerous autophagic vacuoles and dense bodies resembling age pigments were formed in the hepatocytic cytoplasm. Mitochondrial enlargement, increased matrical density and rough endoplasmic reticulum fragmentation were also noted. Few of these changes were observed at 9 weeks, and the hepatocyte's morphology was virtually normal at 15 and 24 weeks. The marked changes seen at 3 weeks may be a manifestation of the body's adaptive processes to the nutritional stress.
测定了自由采食大鼠以及长期饮食限制（分别为自由采食组饮食量的10%、30%和50%）大鼠肝脏匀浆中溶酶体酶酸性磷酸酶、β-半乳糖苷酶、芳基硫酸酯酶B和组织蛋白酶D的比活性。饮食限制在断奶后不久开始，3、9、15和24周后处死动物。饮食限制影响了所有这四种酶，但变化取决于酶的种类以及饮食限制的程度和持续时间。酸性磷酸酶的总活性在限制3周时显著增加，但仅在50%组中出现。9周时活性恢复到正常水平。芳基硫酸酯酶B在所有实验组中均增加，在3周时以及在限制更严格的大鼠中观察到更明显的变化。未观察到β-半乳糖苷酶和组织蛋白酶D活性有明显变化。肝脏超微结构的变化与3周时观察到的生化变化平行。在肝细胞质中形成了许多自噬泡和类似老年色素的致密体。还注意到线粒体增大、基质密度增加和粗面内质网断裂。9周时观察到的这些变化较少，15周和24周时肝细胞形态基本正常。3周时出现的明显变化可能是机体对营养应激的适应性过程的一种表现。
