Ferland G, Perea A, Audet M, Tuchweber B
Department of Nutrition, Universite de Montreal, Quebec, Canada.
Mech Ageing Dev. 1990 Nov;56(2):143-54. doi: 10.1016/0047-6374(90)90005-z.
The specific activity of 4 lysosomal enzymes was studied in homogenate, hepatocytes, Kupffer and endothelial cells isolated from the livers of female Sprague-Dawley rats aged 3.5, 12 and 24 months. Cells were obtained by enzymatic digestion and centrifugal elutriation. Cell viability was not affected by age or diet. In hepatocytes, the activities of all enzymes (acid phosphatase, beta-galactosidase, arylsulfatase B and cathepsin D) increased with age in rats fed ad libitum (A) but were not altered significantly by dietary restriction. The activities of all enzymes except acid phosphatase were systematically higher at 3.5 months of age in Kupffer and endothelial cells than in hepatocytes. Acid phosphatase, arylsulfatase B and cathepsin D activities increased with age in both Kupffer and endothelial cells. Beta galactosidase was decreased significantly with age in Kupffer cells but was elevated in endothelial cells. Rats exposed to dietary restriction (R) showed higher activities of beta-galactosidase, arylsulfatase B and cathepsin D when compared to corresponding A animals with the exception of the younger age group. No clear cut pattern was observed in acid phosphatase activity. Thus, the activities of liver lysosomal enzymes increase with age but the pattern of change differs with respect to enzyme and cell populations. The heightened enzyme activity in Kupffer and endothelial cells from R rats may reflect a more efficient phagocytic capacity in these animals.
对3.5个月、12个月和24个月龄雌性Sprague-Dawley大鼠肝脏匀浆、肝细胞、库普弗细胞和内皮细胞中4种溶酶体酶的比活性进行了研究。细胞通过酶消化和离心淘洗获得。细胞活力不受年龄或饮食的影响。在自由采食（A）的大鼠肝细胞中，所有酶（酸性磷酸酶、β-半乳糖苷酶、芳基硫酸酯酶B和组织蛋白酶D）的活性随年龄增加，但饮食限制对其没有显著影响。在3.5个月龄时，库普弗细胞和内皮细胞中除酸性磷酸酶外的所有酶的活性均系统性地高于肝细胞。库普弗细胞和内皮细胞中酸性磷酸酶、芳基硫酸酯酶B和组织蛋白酶D的活性随年龄增加。库普弗细胞中β-半乳糖苷酶的活性随年龄显著降低，但在内皮细胞中升高。与相应的自由采食（A）动物相比，除较年轻年龄组外，饮食限制（R）大鼠的β-半乳糖苷酶、芳基硫酸酯酶B和组织蛋白酶D的活性更高。酸性磷酸酶活性未观察到明确的变化模式。因此，肝脏溶酶体酶的活性随年龄增加，但变化模式因酶和细胞群体而异。饮食限制（R）大鼠的库普弗细胞和内皮细胞中酶活性升高可能反映了这些动物更有效的吞噬能力。
