Baker R E, Corner M A, Kleiss M
Neurosci Lett. 1983 Nov 11;41(3):321-4. doi: 10.1016/0304-3940(83)90470-6.
The distribution of sensory evoked bioelectric activity was examined in spinal cord-dorsal root ganglion (SC-DRG) preparations cultured in a chemically defined, serum-free medium (CDM). DRG afferents showed no preferential innervation of dorsal cord regions in this CDM, on the basis of electrophysiological mapping of the distribution of evoked responses at 4 weeks in vitro. Addition of chondroitin sulfate, galactose-1-phosphate or D(+)-galactose (but not glucose-1-phosphate) to the CDM resulted in a significant increase in presumed monosynaptic connections within the dorsal cord, thus mimicking the results observed in serum-supplemented medium [1,6]. Inasmuch as D(+)-galactose bears no negative charges yet restores the selective functional innervation, whereas glucose-1-phosphate (a highly charged molecule) fails to do so, it is concluded that it is galactose utilization, rather than the charged nature of the chondroitin sulfate and galactose-1-phosphate molecules, which is responsible for the effect.
在化学限定的无血清培养基（CDM）中培养的脊髓-背根神经节（SC-DRG）制剂中，研究了感觉诱发生物电活动的分布。基于体外4周时诱发反应分布的电生理图谱，在这种CDM中，背根神经节传入神经对脊髓背侧区域没有优先支配。向CDM中添加硫酸软骨素、半乳糖-1-磷酸或D（+）-半乳糖（但不添加葡萄糖-1-磷酸）会导致脊髓背侧假定的单突触连接显著增加，从而模拟了在添加血清的培养基中观察到的结果[1,6]。由于D（+）-半乳糖不带负电荷但能恢复选择性功能支配，而葡萄糖-1-磷酸（一种带高电荷的分子）则不能，因此得出结论，是半乳糖的利用而非硫酸软骨素和半乳糖-1-磷酸分子的带电性质导致了这种效应。
