Dubový P, Malinovský L
Acta Histochem. 1983;73(2):205-18. doi: 10.1016/S0065-1281(83)80029-4.
The ultrastructural localization of alkaline phosphatase has been studied in Pacinian corpuscles of the cat mesentery by the method of Mayahara et al. (1967) with 3 substrates. As control studies, specimens were incubated in the medium containing L-cysteine (10 mmol) or EDTA (5 mmol). The electron opaque final reaction product was observed on plasmic membranes and in cytoplasm and pinocytotic vesicles of the inner core cells. The precipitate was present also in rough endoplasmic reticulum, multivesicular bodies, and cytoplasmic vacuoles of the inner core lamellae. The axon revealed the positive enzymatic activity in the axolemma and the scattered precipitate was found in axoplasm. The pinocytotic vesicles in the capillary endothelium entering Pacinian corpuscles contained the reaction product, too. The capsule lamellae were devoid of precipitate. Localization of alkaline phosphatase in pinocytotic vesicles of the inner core lamellae and capillary wall support the opinion that this enzyme plays the significant role in the phenomenon of the transport of molecules through inner core lamellae from capillaries to the axon in Pacinian corpuscles.
采用Mayahara等人（1967年）的方法，使用3种底物，对猫肠系膜的环层小体中碱性磷酸酶的超微结构定位进行了研究。作为对照研究，将标本在含有L-半胱氨酸（10 mmol）或乙二胺四乙酸（EDTA，5 mmol）的培养基中孵育。在质膜、内芯细胞的细胞质和胞饮小泡中观察到电子不透明的最终反应产物。沉淀物也存在于内芯板层的粗面内质网、多囊体和细胞质空泡中。轴突在轴膜上显示出阳性酶活性，轴质中发现有散在的沉淀物。进入环层小体的毛细血管内皮中的胞饮小泡也含有反应产物。被膜板层没有沉淀物。碱性磷酸酶在内芯板层和毛细血管壁的胞饮小泡中的定位支持了这样一种观点，即该酶在环层小体中分子从毛细血管通过内芯板层向轴突运输的现象中起重要作用。
