Dubový P, Malinovský L
Z Mikrosk Anat Forsch. 1982;96(5):802-16.
The authors studied nCHE activity in Pacinian corpuscles of the mesentery of adult cats and 14-day-old kittens. The localization of nCHE was performed on non-frozen sections in Karnovsky's method (1964) with inhibitor application. The nCHE activity was by means of reaction product deposits always localized on plasmic membranes of inner core lamellae and on the axon axolemma. On capsule lamellae no nCHE activity was found. In inner core cells of Pacinian corpuscles of adult individuals, owing to sporadic occurrence of organelles their relationship to nCHE activity failed to be established. It was possible only in Pacinian corpuscles of 14-day-old kittens, where organelle findings are substantially more frequent and furthermore the lamellae broader, which facilitates the localization of deposits on plasmic membranes. The nCHE activity was proved here on the rough endoplasmic reticulum and on vesicles of the Golgi complex. For the above-mentioned reasons Pacinian corpuscles of kittens are substantially more suitable for the ultrahistochemical localization of enzymes than those of adult cats. The difference in nCHE activity localization, as it was established by Idé and Saito (1980), is probably caused by the different preparation of sections for incubation (cryostat sections were used). The occurrence of nCHE isoenzymes with different binding to the membranes of inner core cells of the Pacinian corpuscle not only renders exact electron-microscopic localization more difficult but it also shows that, similarly as ACHE, nCHE is synthetized in the rough endoplasmic reticulum in soluble oligomeric form. Only after aggregation of these subunits is it bound to the membranous structures of the inner core. The reversibility of this process can explain the irregular findings of the precipitate, mainly on pinocytotic vesicles. The localization of nCHE on inner core cells supports the opinion that inner core lamellae of Pacinian corpuscles are formed by Schwann cells. The nCHE activity found on all inner core lamellae of Pacinian corpuscles from the axon to the capsule lamellae is further evidence for the nonexistence of a special zone of lamellae, the so-called outer core, between the inner core and capsule.
作者研究了成年猫和14日龄小猫肠系膜中帕氏小体的非特异性胆碱酯酶（nCHE）活性。采用卡尔诺夫斯基方法（1964年）并应用抑制剂，在非冷冻切片上进行nCHE的定位。nCHE活性通过反应产物沉积总是定位于内核板层的质膜和轴突轴膜上。在被膜板层上未发现nCHE活性。在成年个体帕氏小体的内核细胞中，由于细胞器的散在出现，未能确定它们与nCHE活性的关系。这仅在14日龄小猫的帕氏小体中是可能的，在那里细胞器的发现更为频繁，而且板层更宽，这便于沉积物在质膜上的定位。在这里，在糙面内质网和高尔基体小泡上证实了nCHE活性。由于上述原因，小猫的帕氏小体比成年猫的帕氏小体更适合用于酶的超组织化学定位。如井出和斋藤（1980年）所确定的，nCHE活性定位的差异可能是由于孵育切片的制备方法不同（使用了低温恒温器切片）。帕氏小体内核细胞的膜上存在不同结合情况的nCHE同工酶，这不仅使精确的电子显微镜定位更加困难，而且还表明，与乙酰胆碱酯酶（ACHE）类似，nCHE以可溶性寡聚体形式在糙面内质网中合成。只有在这些亚基聚集后，它才与内核的膜结构结合。这个过程的可逆性可以解释沉淀物的不规则发现，主要是在胞饮小泡上。nCHE在内核细胞上的定位支持了这样一种观点，即帕氏小体的内核板层是由施万细胞形成的。在帕氏小体从轴突到被膜板层的所有内核板层上发现的nCHE活性，进一步证明在内核和被膜之间不存在所谓的特殊板层区域，即外核。
