Medearis A L, Hensleigh P A, Parks D R, Herzenberg L A
Am J Obstet Gynecol. 1984 Feb 1;148(3):290-5. doi: 10.1016/s0002-9378(84)80070-8.
A study was made of the frequency and amount of fetal hemorrhage into maternal blood during labor and delivery as evidenced by the number of fetal cells present in the maternal circulation immediately after spontaneous vaginal delivery. A sensitive, indirect immunofluorescence was used with fluorescence-activated cell sorter analysis of erythrocytes. All of the 16 Rh-negative mothers studied after vaginal delivery of Rh-positive infants had circulating Rh-positive cells. The mean Rh-positive to Rh-negative erythrocyte ratio was 1:14, 100 in maternal blood, which corresponds to a mean fetal hemorrhage of 156 microliters. The test described is sufficiently sensitive to be used for the study of primary Rh isoimmunization and could be clinically applicable for antepartum screening to determine which patients require Rh immune globulin treatment before delivery.
一项关于分娩过程中胎儿向母体血液内出血的频率和出血量的研究，通过自然阴道分娩后立即在母体循环中出现的胎儿细胞数量来证明。采用了一种敏感的间接免疫荧光法，并对红细胞进行荧光激活细胞分选分析。在16例Rh阴性母亲自然分娩Rh阳性婴儿后进行研究，所有母亲的循环血液中均有Rh阳性细胞。母体血液中Rh阳性与Rh阴性红细胞的平均比例为1:14100，这相当于平均胎儿出血量为156微升。所描述的检测方法足够敏感，可用于原发性Rh同种免疫的研究，并且在临床上可用于产前筛查，以确定哪些患者在分娩前需要接受Rh免疫球蛋白治疗。
