Simeone A, Boncinelli E
FEBS Lett. 1984 Feb 27;167(2):249-53. doi: 10.1016/0014-5793(84)80136-2.
We determined the nucleotide sequence of the DNA region around the 5'-terminus of 18 S rRNA in two cloned rDNA gene units of Drosophila melanogaster. The 5'-base is within a sequence CATTATT which is present also at the 3'-terminus of the 18 S rRNA coding region. In this case it is known that the situation is CATTA3' . TT. With various methods we determined that the precise 5'-cleavage site is CATT . 5'ATT.
我们测定了黑腹果蝇两个克隆的核糖体DNA(rDNA)基因单位中18S核糖体RNA(rRNA)5'端周围DNA区域的核苷酸序列。5'端碱基位于序列CATTATT内,该序列也存在于18S rRNA编码区域的3'端。在这种情况下,已知的情况是CATTA3'。TT。我们用各种方法确定精确的5'切割位点是CATT。5'ATT。