红头丽蝇核糖体DNA的内含子边界及侧翼核糖体RNA编码序列
The intron boundaries and flanking rRNA coding sequences of Calliphora erythrocephala rDNA.
作者信息
Smith V L, Beckingham K
出版信息
Nucleic Acids Res. 1984 Feb 10;12(3):1707-24. doi: 10.1093/nar/12.3.1707.
We have sequenced the available cloned examples of the intron-coding sequence junctions for the rDNA of the higher Dipteran, Calliphora erythrocephala. The introns interrupt the rDNA at the same position as the type 1 intron family detected in Drosophila melanogaster and D. virilis (10,11). A duplication of 14 base pairs of the 28S rRNA coding sequence surrounds a short version of the major genomic length class of introns. This same duplication is associated with boundaries of the type 1 introns in D. virilis and D. melanogaster (10, 13,14). We have detected considerable homology between the 3' intron sequences of C. erythrocephala and D. virilis. The rRNA coding sequences flanking the introns are extremely homologous in C. erythrocephala, D. melanogaster and D. virilis, with only one small region of significant divergence. This corresponds to a variable stem region previously identified in eukaryotic 28S rRNA at a site analogous to the L1 ribosomal protein binding site of prokaryotic 23S rRNA (27).
我们已对双翅目昆虫红头丽蝇（Calliphora erythrocephala）核糖体DNA（rDNA）内含子编码序列连接区的现有克隆样本进行了测序。这些内含子在与黑腹果蝇（Drosophila melanogaster）和拟果蝇（D. virilis）中检测到的1型内含子家族相同的位置打断rDNA。28S rRNA编码序列的14个碱基对的重复序列环绕着主要基因组长度类别的内含子的一个短版本。同样的重复序列与拟果蝇和黑腹果蝇中1型内含子的边界相关（参考文献10、13、14）。我们检测到了红头丽蝇和拟果蝇3'内含子序列之间存在相当大的同源性。内含子两侧的rRNA编码序列在红头丽蝇、黑腹果蝇和拟果蝇中极其同源，只有一个小区域存在显著差异。这对应于先前在真核生物28S rRNA中一个类似于原核生物23S rRNA的L1核糖体蛋白结合位点的位置所鉴定出的可变茎区（参考文献27）。
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