Gorelyshev A S, Tsygankov Iu D, Kul'ba A M
Genetika. 1984 Jan;20(1):185-6.
The temperate bacteriophage SM is not serologically related to the known transducing phages F116, G101, B3 of Pseudomonas aeruginosa. The strains with auxotrophic mutations within the wide ranges of the genetic map of P. aeruginosa strain PAO1 were used for studying the transducing activity of the SM phage. All of the 7 bacterial markers tested are transduced with SM phage grown on a prototrophic donor strain. The frequency of transduction of separate bacterial markers using the wild type SM phage is 2.3 to 4.6 X 10(-8). Linked ilv202+ - met28+ markers are cotransduced with SM phage at a frequency of about 1.5%.
温和噬菌体SM与铜绿假单胞菌已知的转导噬菌体F116、G101、B3没有血清学关系。利用铜绿假单胞菌PAO1菌株遗传图谱广泛范围内具有营养缺陷型突变的菌株来研究SM噬菌体的转导活性。在原养型供体菌株上生长的SM噬菌体可转导所测试的全部7种细菌标记。使用野生型SM噬菌体时,各细菌标记的转导频率为2.3至4.6×10⁻⁸。连锁的ilv202⁺ - met28⁺标记与SM噬菌体的共转导频率约为1.5%。