McMaster P R, Tsang V C, Wong K H, Feeley J C, Gann D S
Int Arch Allergy Appl Immunol. 1984;73(4):357-62. doi: 10.1159/000233498.
Adjuvant activity of heat-killed Legionella pneumophila was demonstrated and compared with that of inactivated Mycobacterium tuberculosis H37Rv. The two species of bacteria were suspended separately in oil and Arlacel A. Bovine serum albumin (BSA) in saline was then emulsified within the respective adjuvants and injected intradermally into guinea pigs. Antibodies to the BSA antigen in the sera of the animals were quantitated with the kinetic-dependent enzyme-linked immunosorbent assay (k-Elisa). Guinea pigs immunized with BSA in adjuvant with killed L. pneumophila produced high titers of anti-BSA antibody, which, on the average, were nearly as high as in those immunized with BSA in complete Freund's adjuvant with M. tuberculosis H37Rv, and which were much greater than in others immunized with incomplete adjuvant, lacking bacteria. Moreover, with a polypeptide hapten, the L. pneumophila evoked as much or more antibody in rabbits as the mycobacterium adjuvant. The effect of the legionella adjuvant upon the cellular immune response was examined using skin tests. For this purpose guinea pigs were immunized with picryl-guinea pig albumin in these adjuvants. 6 weeks later, they were skin-tested with that antigen. They showed reactions which appeared to have immediate as well as delayed components when examined grossly and histologically. Others, immunized with incomplete adjuvant, did not exhibit delayed reactions. Accordingly, heat-killed L. pneumophila acts as a potent adjuvant. Under the circumstances of these experiments, it was as effective as heat-killed M. tuberculosis.
已证实热灭活嗜肺军团菌的佐剂活性，并将其与灭活的结核分枝杆菌H37Rv的佐剂活性进行比较。将这两种细菌分别悬浮于油和阿拉塞尔A中。然后将盐水中的牛血清白蛋白（BSA）在各自的佐剂中乳化，并皮内注射到豚鼠体内。用动力学依赖酶联免疫吸附测定法（k-ELISA）对动物血清中针对BSA抗原的抗体进行定量。用热灭活嗜肺军团菌作为佐剂免疫的豚鼠产生了高滴度的抗BSA抗体，平均而言，其滴度几乎与用结核分枝杆菌H37Rv的完全弗氏佐剂免疫的豚鼠一样高，且远高于用不含细菌的不完全佐剂免疫的其他豚鼠。此外，对于一种多肽半抗原，嗜肺军团菌在兔体内诱发的抗体与分枝杆菌佐剂诱发的一样多或更多。使用皮肤试验研究了军团菌佐剂对细胞免疫反应的影响。为此，用这些佐剂中的苦味酰-豚鼠白蛋白免疫豚鼠。6周后，用该抗原对它们进行皮肤试验。大体和组织学检查显示，它们的反应似乎既有即时成分，也有延迟成分。而用不完全佐剂免疫的其他豚鼠则没有表现出延迟反应。因此，热灭活嗜肺军团菌可作为一种有效的佐剂。在这些实验条件下，它与热灭活结核分枝杆菌一样有效。
