用固相免疫荧光法检测免疫豚鼠血清中嗜肺军团菌抗体
Detection of antibodies to Legionella pneumophila in immune guinea pig serum by solid-phase immunofluorescence.
作者信息
Vogel F R, Klein T W, Specter S C, Hitchings M, Friedman H
出版信息
J Clin Microbiol. 1981 Apr;13(4):726-9. doi: 10.1128/jcm.13.4.726-729.1981.
A semiautomated solid-phase immunofluorescence apparatus (FIAX; International Diagnostic Technology, Santa Clara, Calif.) was utilized to develop a rapid method for detection of antibody to Legionella pneumophila. The sera from guinea pigs immunized with a mixture of killed L. pneumophila and Freund complete adjuvant displayed markedly enhanced antibody activity as measured by FIAX when compared with that obtained from adjuvant-injected or unimmunized animals. A correlation was observed between FIAX net fluorescence units and microagglutination titers of serum samples obtained from immunized animals. Within-run and between-run coefficients of variation performed on selected immune serum samples were low. These results demonstrated that the FIAX method could readily and reproducibly detect Legionella-specific antibodies in the sera of actively immunized animals and suggest the possibility of a broader application of FIAX in the serological detection of exposure to L. pneumophila antigen.
使用半自动固相免疫荧光仪（FIAX；国际诊断技术公司，加利福尼亚州圣克拉拉）开发了一种快速检测嗜肺军团菌抗体的方法。与注射佐剂或未免疫动物相比，用灭活嗜肺军团菌和弗氏完全佐剂混合物免疫的豚鼠血清，通过FIAX检测显示抗体活性明显增强。在免疫动物血清样本中，观察到FIAX净荧光单位与微量凝集效价之间存在相关性。对选定免疫血清样本进行的批内和批间变异系数较低。这些结果表明，FIAX方法可以轻松且可重复地检测主动免疫动物血清中的军团菌特异性抗体，并提示FIAX在嗜肺军团菌抗原暴露血清学检测中有更广泛应用的可能性。
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