Mason J M, Setlow P
J Bacteriol. 1984 Mar;157(3):931-3. doi: 10.1128/jb.157.3.931-933.1984.
The small acid-soluble spore proteins alpha and beta were not detected during stationary-phase growth of asporogenous Bacillus subtilis mutants blocked in stages 0, II, or III, but mutants blocked in stages IV or V accumulated nearly wild-type levels of these small acid-soluble spore proteins. Similar results were obtained when production of Bacillus megaterium C protein (also a small acid-soluble spore protein), as well as alpha and beta, were monitored in these mutants containing a recombinant plasmid carrying the B. megaterium C protein gene. The only exception was a spo0H mutant which synthesized a small amount of C protein, but no alpha or beta.
在处于0期、II期或III期停滞的枯草芽孢杆菌无芽孢突变体的稳定期生长过程中,未检测到小酸溶性芽孢蛋白α和β,但处于IV期或V期停滞的突变体积累了接近野生型水平的这些小酸溶性芽孢蛋白。当在含有携带巨大芽孢杆菌C蛋白基因的重组质粒的这些突变体中监测巨大芽孢杆菌C蛋白(也是一种小酸溶性芽孢蛋白)以及α和β的产生时,也得到了类似的结果。唯一的例外是一个spo0H突变体,它合成了少量的C蛋白,但没有α或β。