巨大芽孢杆菌和枯草芽孢杆菌小酸溶性芽孢蛋白基因在枯草芽孢杆菌无芽孢突变体稳定期生长过程中的表达
Expression of Bacillus megaterium and Bacillus subtilis small acid-soluble spore protein genes during stationary-phase growth of asporogenous B. subtilis mutants.
作者信息
Mason J M, Setlow P
出版信息
J Bacteriol. 1984 Mar;157(3):931-3. doi: 10.1128/jb.157.3.931-933.1984.
Abstract
The small acid-soluble spore proteins alpha and beta were not detected during stationary-phase growth of asporogenous Bacillus subtilis mutants blocked in stages 0, II, or III, but mutants blocked in stages IV or V accumulated nearly wild-type levels of these small acid-soluble spore proteins. Similar results were obtained when production of Bacillus megaterium C protein (also a small acid-soluble spore protein), as well as alpha and beta, were monitored in these mutants containing a recombinant plasmid carrying the B. megaterium C protein gene. The only exception was a spo0H mutant which synthesized a small amount of C protein, but no alpha or beta.
摘要
在处于0期、II期或III期停滞的枯草芽孢杆菌无芽孢突变体的稳定期生长过程中，未检测到小酸溶性芽孢蛋白α和β，但处于IV期或V期停滞的突变体积累了接近野生型水平的这些小酸溶性芽孢蛋白。当在含有携带巨大芽孢杆菌C蛋白基因的重组质粒的这些突变体中监测巨大芽孢杆菌C蛋白（也是一种小酸溶性芽孢蛋白）以及α和β的产生时，也得到了类似的结果。唯一的例外是一个spo0H突变体，它合成了少量的C蛋白，但没有α或β。
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