Gingrich J C, Lundell D J, Glazer A N
J Cell Biochem. 1983;22(1):1-14. doi: 10.1002/jcb.240220102.
The tricylindrical core of Synechocystis 6701 phycobilisomes is made up of four types of allophycocyanin-containing complexes: A, (alpha AP beta AP)3; B, (alpha AP beta AP)3 .10K; C, (alpha APB1 alpha AP2 beta AP3).10K; D, (alpha AP beta AP)2.18.5K.99K; where AP is allophycocyanin, APB is allophycocyanin B, and 10K, 18.5K, and 99K are polypeptides of 10,000, 18,500, and 99,000 daltons, respectively. The 18.5K polypeptide is a hitherto unrecognized biliprotein subunit with a single phycocyanobilin prosthetic group. The tricylindrical core is made up of 12 subcomplexes in the molar ratio of A:B:C:D: of 4:4:2:2. Complexes C and D act as terminal energy acceptors. From these results and previous analysis of the bicylindrical core of Synechococcus 6301 phycobilisomes [14,15] it is proposed that the two cylinders of the Synechocystis 6701 core, proximal to the thylakoid membrane, each have the composition ABCD, and that the distal cylinder has the composition A2B2.
集胞藻6701藻胆体的三圆柱形核心由四种含别藻蓝蛋白的复合物组成:A,(αAPβAP)3;B,(αAPβAP)3·10K;C,(αAPB1αAP2βAP3)·10K;D,(αAPβAP)2·18.5K·99K;其中AP是别藻蓝蛋白,APB是别藻蓝蛋白B,10K、18.5K和99K分别是分子量为10000、18500和99000道尔顿的多肽。18.5K多肽是一种迄今未被识别的双蛋白亚基,带有单个藻蓝胆素辅基。三圆柱形核心由12个亚复合物组成,其摩尔比为A:B:C:D = 4:4:2:2。复合物C和D作为末端能量受体。根据这些结果以及之前对聚球藻6301藻胆体双圆柱形核心的分析[14,15],有人提出集胞藻6701核心靠近类囊体膜的两个圆柱,每个都具有ABCD的组成,而远端圆柱具有A2B2的组成。