Anderson L K, Rayner M C, Eiserling F A
J Bacteriol. 1987 Jan;169(1):102-9. doi: 10.1128/jb.169.1.102-109.1987.
The unicellular cyanobacterium Synechocystis sp. strain 6701 was mutagenized with UV irradiation and screened for pigment changes that indicated genetic lesions involving the light-harvesting proteins of the phycobilisome. A previous examination of the pigment mutant UV16 showed an assembly defect in the phycocyanin component of the phycobilisome. Mutagenesis of UV16 produced an additional double mutant, UV16-40, with decreased phycoerythrin content. Phycocyanin and phycoerythrin were isolated from UV16-40 and compared with normal biliproteins. The results suggested that the UV16 mutation affected the alpha subunit of phycocyanin, while the phycoerythrin beta subunit from UV16-40 had lost one of its three chromophores. Characterization of the unassembled phycobilisome components in these mutants suggests that these strains will be useful for probing in vivo the regulated expression and assembly of phycobilisomes.
单细胞蓝藻聚球藻属6701菌株经紫外线照射诱变处理，并筛选表明涉及藻胆体捕光蛋白的遗传损伤的色素变化。之前对色素突变体UV16的检测显示藻胆体的藻蓝蛋白组分存在组装缺陷。UV16诱变产生了另一个双突变体UV16-40，其藻红蛋白含量降低。从UV16-40中分离出藻蓝蛋白和藻红蛋白，并与正常的胆色素蛋白进行比较。结果表明，UV16突变影响藻蓝蛋白的α亚基，而UV16-40的藻红蛋白β亚基失去了其三个发色团之一。对这些突变体中未组装的藻胆体组分的表征表明，这些菌株将有助于在体内探究藻胆体的调控表达和组装。
