使用简易反射仪测量血氨。

Blood ammonia measurement using a simple reflectometer.

作者信息

Ratnaike R N, Buttery J E, Hoffmann S

出版信息

J Clin Chem Clin Biochem. 1984 Jan;22(1):105-8. doi: 10.1515/cclm.1984.22.1.105.

DOI:10.1515/cclm.1984.22.1.105

PMID:6421986

Abstract

We have assessed a compact Blood Ammonia Checker System (Ammonia Checker) consisting of a reflectometer which measures the intensity of colour formed by blood ammonia on a bromocresol green indicator reagent plate. The results show good correlation (r = 0.95) with our routine chemical method and a regression line y = 0.835X - 1.468. The Ammonia Checker has good precision (CV less than 10%) at the critical blood ammonia concentration and accurately measures predetermined ammonia concentrations. Blood sampling is improved with a precision pipette. The Ammonia Checker is simple, convenient and reliable and is ideally suited for a laboratory required to perform an urgent blood ammonia measurement.

摘要

我们评估了一种紧凑型血氨检测系统（氨检测仪），该系统由一台反射光度计组成，用于测量血氨在溴甲酚绿指示试剂板上形成的颜色强度。结果显示，其与我们的常规化学方法具有良好的相关性（r = 0.95），回归方程为y = 0.835X - 1.468。氨检测仪在临界血氨浓度下具有良好的精密度（变异系数小于10%），并能准确测量预定的氨浓度。使用精密移液器可改善血液采样。氨检测仪操作简单、方便且可靠，非常适合需要进行紧急血氨测量的实验室。