Mohammad A R
J Oral Pathol. 1984 Feb;13(1):16-21. doi: 10.1111/j.1600-0714.1984.tb01396.x.
Stimulation of cell-mediated immunity (CMI) by 2,4 dinitrochlorobenzene (DNCB) was reported to be effective in clinical regression of some carcinomas and precancerous lesions. This study investigated development of CMI in rat submaxillary salivary glands with dermal application of DNCB to provide a model for the study of immunotherapy in salivary gland neoplasia. Twenty rats received 0.02 ml of 0.5% DNCB in 4:1 acetone corn-oil applied with a glass rod to 3 cm2 of clipped ventral skin of the neck covering the submaxillary salivary gland on 2 successive days. Ten days later, the submaxillary glands were challenged with an injection of 0.02 ml of DNCB. Thirty-two h later the animals were killed and the glands examined grossly and microscopically. Thirty control animals were sensitized and challenged, (a) 10 with 0.5% DNCB, then vehicle only, (b) 10 with vehicle only, then 0.5% DNCB, and (c) 10 with vehicle both times. Gross examination of experimental and control glands disclosed moderate edema and congestion. Microscopically, experimental glands showed inflammatory changes including infiltration, vascular congestion and perivascular cuffing of lymphocytes. Regional lymph nodes showed proliferation of cortical germinal centers only. Control glands showed acute vascular congestion in group (a), and absence of microscopic features of delayed hypersensitivity.
据报道，2,4-二硝基氯苯（DNCB）刺激细胞介导免疫（CMI）对某些癌症和癌前病变的临床消退有效。本研究通过在大鼠颌下唾液腺皮肤局部应用DNCB来研究CMI的发展情况，以提供唾液腺肿瘤免疫治疗研究的模型。20只大鼠连续两天用玻璃棒将0.02ml含4:1丙酮玉米油的0.5% DNCB涂抹于颈部腹侧修剪过的3cm²皮肤处，该区域覆盖颌下唾液腺。10天后，给颌下腺注射0.02ml DNCB进行激发。32小时后处死动物，对腺体进行大体和显微镜检查。30只对照动物进行致敏和激发，（a）10只用0.5% DNCB致敏，然后只用赋形剂；（b）10只用赋形剂致敏，然后用0.5% DNCB；（c）10只两次都用赋形剂。对实验和对照腺体的大体检查显示有中度水肿和充血。显微镜下，实验腺体出现炎症变化，包括淋巴细胞浸润、血管充血和血管周围套袖现象。局部淋巴结仅显示皮质生发中心增生。对照腺体在（a）组显示急性血管充血，且无迟发型超敏反应的显微镜特征。
