Obalek S, Haftek M, Sliński W
Dermatologica. 1977;155(1):13-25.
Quantitative techniques of sensitization to 2,4-dinitrochlorobenzene (DNCB) was used to determine in psoriasis the intensity and frequency of allergic reactions to DNCB following primary challenge with 2,000 microgram allergen and secondary challenge with decreasing doses of DNCB. 56 patients with psoriasis and 23 healthy volunteers were examined. Frequency of positive reactions to DNCB was similar in both groups, since all normal controls were sensitized, whereas only 8 of 56 psoriasis cases failed to develop delayed hypersensitivity to DNCB. However, the intensity of acquired contact allergy was significantly diminished in psoriasis in comparison with controls. The patients with stationary skin lesions resembled the normal population in the intensity of reaction to DNCB. Decreased intensity of DNCB sensitization seemed to be related to the activity of the disease, but not correlated with the extent of the lesions. A relationship was found between reduced reactivity to DNCB and decrease in E rosette-forming lymphocytes. The data suggest that the impaired function of T lymphocytes in active psoriasis could be responsible for both, defective recognition of contact antigens, such as DNCB, and the alteration of secondary response to DNCB.
采用对2,4 - 二硝基氯苯（DNCB）致敏的定量技术，以确定银屑病患者在初次用2000微克变应原激发以及随后用递减剂量的DNCB再次激发后，对DNCB过敏反应的强度和频率。对56例银屑病患者和23名健康志愿者进行了检查。两组对DNCB阳性反应的频率相似，因为所有正常对照均已致敏，而56例银屑病患者中只有8例对DNCB未产生迟发型超敏反应。然而，与对照组相比，银屑病患者获得性接触性过敏的强度显著降低。皮肤损害静止的患者对DNCB的反应强度与正常人群相似。DNCB致敏强度降低似乎与疾病活动有关，但与皮损范围无关。发现对DNCB反应性降低与E玫瑰花结形成淋巴细胞减少之间存在关联。数据表明，活动性银屑病中T淋巴细胞功能受损可能是对接触性抗原（如DNCB）识别缺陷以及对DNCB二次反应改变的原因。
