Kato Y, Kanno T
Regul Pept. 1983 Dec;7(4):347-56. doi: 10.1016/0167-0115(83)90106-4.
The effects of intracerebroventricular (i.c.v.) injection of synthetic thyrotropin-releasing hormone (TRH) and its analogue (gamma-butyrolactone-gamma-carbonyl-His-Pro-NH2) were tested in anesthetized rats fitted with pancreatic cannula. TRH injection induced dose-related increases in flow of pancreatic juice, protein output, and amylase output, each reaching a maximum within 10 min. Higher doses of TRH induced longer responses. Injection of the TRH analogue also caused dose-related secretory responses of the exocrine pancreas. The dose-related secretory responses to TRH and the TRH analogue were similar except that the responses to the highest dose of TRH analogue (1600 pmol/100 g b.w.) were significantly higher. Intravenous injection of TRH and the TRH analogue induced little, if any, secretory response of the exocrine pancreas. The effects of i.c.v. injection of TRH and the TRH analogue were completely abolished after bilateral subdiaphragmatic vagotomy. In addition to the secretory effects on the exocrine pancreas, i.c.v. injection of TRH and the analogue caused hyperglycemia, tachycardia, and tear secretion, but the intravenous injection of these peptides had no effect.
在装有胰腺插管的麻醉大鼠中，测试了脑室内（i.c.v.）注射合成促甲状腺激素释放激素（TRH）及其类似物（γ-丁内酯-γ-羰基-His-Pro-NH2）的效果。注射TRH可引起胰液分泌量、蛋白质分泌量和淀粉酶分泌量呈剂量相关增加，每种分泌量在10分钟内达到最大值。更高剂量的TRH可诱导更长时间的反应。注射TRH类似物也引起外分泌胰腺的剂量相关分泌反应。对TRH和TRH类似物的剂量相关分泌反应相似，只是对最高剂量TRH类似物（1600 pmol/100 g体重）的反应明显更高。静脉注射TRH和TRH类似物对外分泌胰腺几乎没有分泌反应（如果有的话）。双侧膈下迷走神经切断术后，脑室内注射TRH和TRH类似物的效果完全消失。除了对外分泌胰腺的分泌作用外，脑室内注射TRH和类似物还会导致高血糖、心动过速和泪液分泌，但静脉注射这些肽则没有效果。
