Höhne M, Bayer D, Prehn S, Schewe T, Rapoport S M
Biomed Biochim Acta. 1983;42(9):1129-34.
Lipoxygenase activity with linoleic acid as substrate and the immunologically detectable amount of lipoxygenase protein were estimated in the course of in vitro maturation of rabbit reticulocytes withdrawn at the sixth day of an experimental bleeding anaemia. With unseparated cell mixture there was a significant increase in the lipoxygenase activity of 67 +/- 15% after a maturation period of 4 h followed by a decrease up to the initial level. The maturational changes were more pronounced when the fraction of youngest reticulocytes after buoyant density separation in a serum albumin gradient was used, whereas the cells of medium density failed to show the intermittent increase. The lipoxygenase activity was largely paralleled by the amount of lipoxygenase protein. The increase of lipoxygenase was prevented by either anaerobiosis or addition of oligomycin. The protein synthesis was greatly decreased after 4 h of incubation. The decline of the amount of lipoxygenase between 4 and 24 h incubation is probably largely caused by proteolysis. The results support former interpretations as to the synthesis and biological dynamics of lipoxygenase in reticulocytes.
以亚油酸为底物测定了兔网织红细胞在实验性出血性贫血第6天抽取后进行体外成熟过程中的脂氧合酶活性，并测定了可通过免疫检测到的脂氧合酶蛋白量。对于未分离的细胞混合物，在4小时的成熟培养期后，脂氧合酶活性显著增加67±15%，随后下降至初始水平。当使用在血清白蛋白梯度中通过浮力密度分离得到的最年轻网织红细胞部分时，成熟变化更为明显，而中等密度的细胞未显示出这种间歇性增加。脂氧合酶活性在很大程度上与脂氧合酶蛋白量平行。厌氧或添加寡霉素可阻止脂氧合酶的增加。孵育4小时后蛋白质合成大幅下降。在4至24小时孵育期间脂氧合酶量的下降可能主要是由蛋白水解引起的。这些结果支持了先前关于网织红细胞中脂氧合酶的合成和生物学动态的解释。
